Diagnosis of tuberculosis from different body fluids remains challenging due to various limitations of the conventional and molecular methods. We studied the role of adenosine deaminase (ADA) assay to diagnose tubercular infection in cerebrospinal fluid, peritoneal fluid and pleural fluid. Fifty three patients with tubercular meningitis, peritonitis and pleuritis were enrolled in this study on the basis of clinical, radiological, cytological, biochemical and somewhere bacteriological evidences. Cases positive by AFB smear, culture or PCR were considered as confirmed TB and other as probable TB cases. Another 28 non-TB cases were included as control. In 53 suspected TB cases ADA was found positive in highest 42 (79.2%) cases, whereas smear and/ culture in 10 (18.7%) and PCR in 18 (33.9%) cases. ADA assay revealed 100% positivity in confirmed TB cases and 14.3% in non TB cases. The sensitivity and specificity of ADA was found 79% and 86% respectively when the cut off value was used ≥ 10 IU/L for CSF and ≥ 40 IU/L for pleural or peritoneal fluid. Mean ADA values of confirmed and probable TB cases were found significantly higher than that of non TB cases (< 0.05). Significance of difference was determined by ANOVA and Kruskal-Wallis test. Thus, adenosine deaminase assay in body fluids has proved to be efficient, reliable and simple method to diagnose tubercular meningitis, peritonitis and pleuritis.
| Published in | American Journal of Biomedical and Life Sciences (Volume 3, Issue 3) |
| DOI | 10.11648/j.ajbls.20150303.14 |
| Page(s) | 49-54 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Body Fluid, Tuberculosis, Adenosine Deaminase Assay
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APA Style
Khandaker Shadia, S. M. Mostofa Kamal, Ahmed Abu Saleh, Mohammed Nayem Hossain, Ratan Das Gupta, et al. (2015). Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. American Journal of Biomedical and Life Sciences, 3(3), 49-54. https://doi.org/10.11648/j.ajbls.20150303.14
ACS Style
Khandaker Shadia; S. M. Mostofa Kamal; Ahmed Abu Saleh; Mohammed Nayem Hossain; Ratan Das Gupta, et al. Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. Am. J. Biomed. Life Sci. 2015, 3(3), 49-54. doi: 10.11648/j.ajbls.20150303.14
AMA Style
Khandaker Shadia, S. M. Mostofa Kamal, Ahmed Abu Saleh, Mohammed Nayem Hossain, Ratan Das Gupta, et al. Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. Am J Biomed Life Sci. 2015;3(3):49-54. doi: 10.11648/j.ajbls.20150303.14
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Download@article{10.11648/j.ajbls.20150303.14,
author = {Khandaker Shadia and S. M. Mostofa Kamal and Ahmed Abu Saleh and Mohammed Nayem Hossain and Ratan Das Gupta and Md. Ruhul Amin Miah},
title = {Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection},
journal = {American Journal of Biomedical and Life Sciences},
volume = {3},
number = {3},
pages = {49-54},
doi = {10.11648/j.ajbls.20150303.14},
url = {https://doi.org/10.11648/j.ajbls.20150303.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150303.14},
abstract = {Diagnosis of tuberculosis from different body fluids remains challenging due to various limitations of the conventional and molecular methods. We studied the role of adenosine deaminase (ADA) assay to diagnose tubercular infection in cerebrospinal fluid, peritoneal fluid and pleural fluid. Fifty three patients with tubercular meningitis, peritonitis and pleuritis were enrolled in this study on the basis of clinical, radiological, cytological, biochemical and somewhere bacteriological evidences. Cases positive by AFB smear, culture or PCR were considered as confirmed TB and other as probable TB cases. Another 28 non-TB cases were included as control. In 53 suspected TB cases ADA was found positive in highest 42 (79.2%) cases, whereas smear and/ culture in 10 (18.7%) and PCR in 18 (33.9%) cases. ADA assay revealed 100% positivity in confirmed TB cases and 14.3% in non TB cases. The sensitivity and specificity of ADA was found 79% and 86% respectively when the cut off value was used ≥ 10 IU/L for CSF and ≥ 40 IU/L for pleural or peritoneal fluid. Mean ADA values of confirmed and probable TB cases were found significantly higher than that of non TB cases (< 0.05). Significance of difference was determined by ANOVA and Kruskal-Wallis test. Thus, adenosine deaminase assay in body fluids has proved to be efficient, reliable and simple method to diagnose tubercular meningitis, peritonitis and pleuritis.},
year = {2015}
}
TY - JOUR T1 - Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection AU - Khandaker Shadia AU - S. M. Mostofa Kamal AU - Ahmed Abu Saleh AU - Mohammed Nayem Hossain AU - Ratan Das Gupta AU - Md. Ruhul Amin Miah Y1 - 2015/04/30 PY - 2015 N1 - https://doi.org/10.11648/j.ajbls.20150303.14 DO - 10.11648/j.ajbls.20150303.14 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 49 EP - 54 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20150303.14 AB - Diagnosis of tuberculosis from different body fluids remains challenging due to various limitations of the conventional and molecular methods. We studied the role of adenosine deaminase (ADA) assay to diagnose tubercular infection in cerebrospinal fluid, peritoneal fluid and pleural fluid. Fifty three patients with tubercular meningitis, peritonitis and pleuritis were enrolled in this study on the basis of clinical, radiological, cytological, biochemical and somewhere bacteriological evidences. Cases positive by AFB smear, culture or PCR were considered as confirmed TB and other as probable TB cases. Another 28 non-TB cases were included as control. In 53 suspected TB cases ADA was found positive in highest 42 (79.2%) cases, whereas smear and/ culture in 10 (18.7%) and PCR in 18 (33.9%) cases. ADA assay revealed 100% positivity in confirmed TB cases and 14.3% in non TB cases. The sensitivity and specificity of ADA was found 79% and 86% respectively when the cut off value was used ≥ 10 IU/L for CSF and ≥ 40 IU/L for pleural or peritoneal fluid. Mean ADA values of confirmed and probable TB cases were found significantly higher than that of non TB cases (< 0.05). Significance of difference was determined by ANOVA and Kruskal-Wallis test. Thus, adenosine deaminase assay in body fluids has proved to be efficient, reliable and simple method to diagnose tubercular meningitis, peritonitis and pleuritis. VL - 3 IS - 3 ER -
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