Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers

Ezzat M. E.; Shabana I. I.; Esawy A. M.; Elsotohy M. E.

doi:doi:10.11648/j.avs.20140206.16

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Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers

Ezzat M. E., Shabana I. I., Esawy A. M., Elsotohy M. E.

Published in Animal and Veterinary Sciences (Volume 2, Issue 6)

Received: 21 October 2014 Accepted: 10 November 2014 Published: 2 December 2014

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Abstract

This study was conducted to determine the prevalence of Salmonellae in broilers farms in Dakahlia Governorate, Egypt. A total of 1000 samples were collected from 200 broiler chickens (40 apparently healthy, 80 diseased chickens and 80 freshly dead broiler chickens).The samples were liver, caecum, heart blood, spleen & kidney. The colonial morphology, microscopical and biochemical identifications of the isolates revealed the presence of 37 out of 200 chickens (18.5%) salmonella species isolates, representing: 3 from apparently healthy chicken (7.5%), 21 from diseased chickens (26.25%) and 13 from freshly dead broiler chickens (16.25%).The rate of recovery of Salmonellae from the different internal organs showed that high recovery rate was from liver, caecum, spleen, heart then kidney as the follow (9.5%), (5.5%), (4.5%), (3%) and (2%), respectively. The serotyping of the isolated salmonellae from chickens were eight S. enteritidis, one S. maccles Field, two S. wingrove, one S. eingedi, three S. rissen,two S. derby, two S. vejle, one S. magherafelt, two S. berta, two S. enterica sub.spp salamae, one S. gueuletapee, one S. blegdam, five S. kentucky, two S. newport, two S. agona and two S. virchow were isolated from broilers. PCR assay was carried out for six serovars (S. enteritidis, S. maccles Field, S. rissen, S. derby, S. magherafelt and S. enterica sub.spp salamae) to detect the presence of invA, sopB and stn gene, All serovars had the three genes. Gentamycin, ciprofloxacin, colistin sulphate and enrofloxacin were found to be the most effective antimicrobials against the tested isolates; while a high resistance to erythromycin and flumequine were shown. High prevalence of Salmonella in broilers and multidrug resistance, constituting a major concern for public health. Further surveillance programs and research are a necessity to understand their epidemiology and to limit the spread of multidrug-resistant Salmonella spp.

Published in	Animal and Veterinary Sciences (Volume 2, Issue 6)
DOI	10.11648/j.avs.20140206.16
Page(s)	189-193
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Salmonella, Broiler, Serotypes, Virulence Genes

References

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APA Style

Ezzat M. E., Shabana I. I., Esawy A. M., Elsotohy M. E. (2014). Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers. Animal and Veterinary Sciences, 2(6), 189-193. https://doi.org/10.11648/j.avs.20140206.16

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ACS Style

Ezzat M. E.; Shabana I. I.; Esawy A. M.; Elsotohy M. E. Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers. Anim. Vet. Sci. 2014, 2(6), 189-193. doi: 10.11648/j.avs.20140206.16

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AMA Style

Ezzat M. E., Shabana I. I., Esawy A. M., Elsotohy M. E. Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers. Anim Vet Sci. 2014;2(6):189-193. doi: 10.11648/j.avs.20140206.16

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@article{10.11648/j.avs.20140206.16,
  author = {Ezzat M. E. and Shabana I. I. and Esawy A. M. and Elsotohy M. E.},
  title = {Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers},
  journal = {Animal and Veterinary Sciences},
  volume = {2},
  number = {6},
  pages = {189-193},
  doi = {10.11648/j.avs.20140206.16},
  url = {https://doi.org/10.11648/j.avs.20140206.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140206.16},
  abstract = {This study was conducted to determine the prevalence of Salmonellae in broilers farms in Dakahlia Governorate, Egypt. A total of 1000 samples were collected from 200 broiler chickens (40 apparently healthy, 80 diseased chickens and 80 freshly dead broiler chickens).The samples were liver, caecum, heart blood, spleen & kidney. The colonial morphology, microscopical and biochemical identifications of the isolates revealed the presence of 37 out of 200 chickens (18.5%) salmonella species isolates, representing: 3 from apparently healthy chicken (7.5%), 21 from diseased chickens (26.25%) and 13 from freshly dead broiler chickens (16.25%).The rate of recovery of Salmonellae from the different internal organs showed that high recovery rate was from liver, caecum, spleen, heart then kidney as the follow (9.5%), (5.5%), (4.5%), (3%) and (2%), respectively. The serotyping of the isolated salmonellae from chickens were eight S. enteritidis, one S. maccles Field, two S. wingrove, one S. eingedi, three S. rissen,two S. derby, two S. vejle, one S. magherafelt, two S. berta, two S. enterica sub.spp salamae, one S. gueuletapee, one S. blegdam, five S. kentucky, two S. newport, two S. agona and two S. virchow were isolated from broilers. PCR assay was carried out for six serovars (S. enteritidis, S. maccles Field, S. rissen, S. derby, S. magherafelt and S. enterica sub.spp salamae) to detect the presence of invA, sopB and stn gene, All serovars had the three genes. Gentamycin, ciprofloxacin, colistin sulphate and enrofloxacin were found to be the most effective antimicrobials against the tested isolates; while a high resistance to erythromycin and flumequine were shown. High prevalence of Salmonella in broilers and multidrug resistance, constituting a major concern for public health. Further surveillance programs and research are a necessity to understand their epidemiology and to limit the spread of multidrug-resistant Salmonella spp.},
 year = {2014}
}

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TY - JOUR
T1 - Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers
AU - Ezzat M. E.
AU - Shabana I. I.
AU - Esawy A. M.
AU - Elsotohy M. E.
Y1 - 2014/12/02
PY - 2014
N1 - https://doi.org/10.11648/j.avs.20140206.16
DO - 10.11648/j.avs.20140206.16
T2 - Animal and Veterinary Sciences
JF - Animal and Veterinary Sciences
JO - Animal and Veterinary Sciences
SP - 189
EP - 193
PB - Science Publishing Group
SN - 2328-5850
UR - https://doi.org/10.11648/j.avs.20140206.16
AB - This study was conducted to determine the prevalence of Salmonellae in broilers farms in Dakahlia Governorate, Egypt. A total of 1000 samples were collected from 200 broiler chickens (40 apparently healthy, 80 diseased chickens and 80 freshly dead broiler chickens).The samples were liver, caecum, heart blood, spleen & kidney. The colonial morphology, microscopical and biochemical identifications of the isolates revealed the presence of 37 out of 200 chickens (18.5%) salmonella species isolates, representing: 3 from apparently healthy chicken (7.5%), 21 from diseased chickens (26.25%) and 13 from freshly dead broiler chickens (16.25%).The rate of recovery of Salmonellae from the different internal organs showed that high recovery rate was from liver, caecum, spleen, heart then kidney as the follow (9.5%), (5.5%), (4.5%), (3%) and (2%), respectively. The serotyping of the isolated salmonellae from chickens were eight S. enteritidis, one S. maccles Field, two S. wingrove, one S. eingedi, three S. rissen,two S. derby, two S. vejle, one S. magherafelt, two S. berta, two S. enterica sub.spp salamae, one S. gueuletapee, one S. blegdam, five S. kentucky, two S. newport, two S. agona and two S. virchow were isolated from broilers. PCR assay was carried out for six serovars (S. enteritidis, S. maccles Field, S. rissen, S. derby, S. magherafelt and S. enterica sub.spp salamae) to detect the presence of invA, sopB and stn gene, All serovars had the three genes. Gentamycin, ciprofloxacin, colistin sulphate and enrofloxacin were found to be the most effective antimicrobials against the tested isolates; while a high resistance to erythromycin and flumequine were shown. High prevalence of Salmonella in broilers and multidrug resistance, constituting a major concern for public health. Further surveillance programs and research are a necessity to understand their epidemiology and to limit the spread of multidrug-resistant Salmonella spp.
VL - 2
IS - 6
ER -

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Author Information

Ezzat M. E.

Faculty of Veterinary medicine, Department of Bacteriology, Immunology and mycology, Suez Canal University, Ismailia, Egypt
Shabana I. I.

Faculty of Veterinary medicine, Department of Bacteriology, Immunology and mycology, Suez Canal University, Ismailia, Egypt
Esawy A. M.

Animal Health Research Institute, Mansoura, Dakahlia
Elsotohy M. E.

Animal Health Research Institute, Mansoura, Dakahlia

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APA Style

Ezzat M. E., Shabana I. I., Esawy A. M., Elsotohy M. E. (2014). Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers. Animal and Veterinary Sciences, 2(6), 189-193. https://doi.org/10.11648/j.avs.20140206.16

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ACS Style

Ezzat M. E.; Shabana I. I.; Esawy A. M.; Elsotohy M. E. Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers. Anim. Vet. Sci. 2014, 2(6), 189-193. doi: 10.11648/j.avs.20140206.16

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AMA Style

Ezzat M. E., Shabana I. I., Esawy A. M., Elsotohy M. E. Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers. Anim Vet Sci. 2014;2(6):189-193. doi: 10.11648/j.avs.20140206.16

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@article{10.11648/j.avs.20140206.16,
  author = {Ezzat M. E. and Shabana I. I. and Esawy A. M. and Elsotohy M. E.},
  title = {Detection of Virulence Genes in Salmonella Serovars Isolated from Broilers},
  journal = {Animal and Veterinary Sciences},
  volume = {2},
  number = {6},
  pages = {189-193},
  doi = {10.11648/j.avs.20140206.16},
  url = {https://doi.org/10.11648/j.avs.20140206.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140206.16},
  abstract = {This study was conducted to determine the prevalence of Salmonellae in broilers farms in Dakahlia Governorate, Egypt. A total of 1000 samples were collected from 200 broiler chickens (40 apparently healthy, 80 diseased chickens and 80 freshly dead broiler chickens).The samples were liver, caecum, heart blood, spleen & kidney. The colonial morphology, microscopical and biochemical identifications of the isolates revealed the presence of 37 out of 200 chickens (18.5%) salmonella species isolates, representing: 3 from apparently healthy chicken (7.5%), 21 from diseased chickens (26.25%) and 13 from freshly dead broiler chickens (16.25%).The rate of recovery of Salmonellae from the different internal organs showed that high recovery rate was from liver, caecum, spleen, heart then kidney as the follow (9.5%), (5.5%), (4.5%), (3%) and (2%), respectively. The serotyping of the isolated salmonellae from chickens were eight S. enteritidis, one S. maccles Field, two S. wingrove, one S. eingedi, three S. rissen,two S. derby, two S. vejle, one S. magherafelt, two S. berta, two S. enterica sub.spp salamae, one S. gueuletapee, one S. blegdam, five S. kentucky, two S. newport, two S. agona and two S. virchow were isolated from broilers. PCR assay was carried out for six serovars (S. enteritidis, S. maccles Field, S. rissen, S. derby, S. magherafelt and S. enterica sub.spp salamae) to detect the presence of invA, sopB and stn gene, All serovars had the three genes. Gentamycin, ciprofloxacin, colistin sulphate and enrofloxacin were found to be the most effective antimicrobials against the tested isolates; while a high resistance to erythromycin and flumequine were shown. High prevalence of Salmonella in broilers and multidrug resistance, constituting a major concern for public health. Further surveillance programs and research are a necessity to understand their epidemiology and to limit the spread of multidrug-resistant Salmonella spp.},
 year = {2014}
}

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