Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days

Karim Christina Scopinho Furquim; Maria Izabel Camargo Mathias; Gislaine Cristina Roma; Letícia Maria Gráballos Ferraz Hebling; Gervásio Henrique Bechara

doi:doi:10.11648/j.avs.20140206.17

Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days

Karim Christina Scopinho Furquim, Maria Izabel Camargo Mathias, Gislaine Cristina Roma, Letícia Maria Gráballos Ferraz Hebling, Gervásio Henrique Bechara

Published in Animal and Veterinary Sciences (Volume 2, Issue 6)

Received: 11 November 2014 Accepted: 28 November 2014 Published: 15 December 2014

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Abstract

A histochemical analysis was performed in this study in order to detect proteins, polysaccharides, lipids, acid phosphatase and calcium in the secretion produced by the salivary glands of Rhipicephalus sanguineus females (Latreille, 1806) (Acari, Ixodidae) fed for 2, 4 and 6 days in New Zealand White rabbits which had been previously immunized with glandular extract obtained from females fed for 4 days (SGE4). The results revealed that such glands presented alterations in their secretory cycle, which occurred: a) by the inactivity of some c1 cells (in the glands of females fed for 2 days) and c4 (in those fed for 4 days) and b) by the modification in the constitution of the secretion of females fed for 2-6 days. It was verified that, in the glands of females fed for 2 days, there was an increase in proteins and calcium; a reduction in lipids and the contents of polysaccharides and acid phosphatase remained unaltered. In those fed for 4 days there was an increase in proteins, calcium and acid phosphatase; reduction in the lipids, and the content of polysaccharides remained unaltered. In the females fed for 6 days an increase in the components was observed; however, there was a reduction in lipids and acid phosphatase. In addition, it was verified that, in a decrescent order of histochemical alterations, the most affected cells were: f; c2, c3, c5; a, d and c4, e in the glands of females fed for 2 days; c5; a, c2, c3, d and c1, e in the glands of females fed for 4 days and a, c1, c2, d and e, c3 in the glands of females fed for 6 days. The data here obtained clearly show that the most pronounced histochemical modifications were detected in the glands of females fed for 2 and 6 days; however, the modifications observed in the females fed for 4 days must also be considered, once they were significant as well. This has probably occurred because the hosts were inoculated with SGE4, obtained from the salivary glands of females fed for 4 days, intermediate stage of the glandular cycle, which contains antigens that are common to glandular tissues of females fed for 2 and 6 days.

Published in	Animal and Veterinary Sciences (Volume 2, Issue 6)
DOI	10.11648/j.avs.20140206.17
Page(s)	194-207
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Rhipicephalus sanguineus, Immunization, Antigen, Salivary Gland, Secretory Cycle, Histochemistry, Immune-Inflammatory Response

References

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Karim Christina Scopinho Furquim, Maria Izabel Camargo Mathias, Gislaine Cristina Roma, Letícia Maria Gráballos Ferraz Hebling, Gervásio Henrique Bechara. (2014). Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days. Animal and Veterinary Sciences, 2(6), 194-207. https://doi.org/10.11648/j.avs.20140206.17

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Karim Christina Scopinho Furquim; Maria Izabel Camargo Mathias; Gislaine Cristina Roma; Letícia Maria Gráballos Ferraz Hebling; Gervásio Henrique Bechara. Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days. Anim. Vet. Sci. 2014, 2(6), 194-207. doi: 10.11648/j.avs.20140206.17

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Karim Christina Scopinho Furquim, Maria Izabel Camargo Mathias, Gislaine Cristina Roma, Letícia Maria Gráballos Ferraz Hebling, Gervásio Henrique Bechara. Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days. Anim Vet Sci. 2014;2(6):194-207. doi: 10.11648/j.avs.20140206.17

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@article{10.11648/j.avs.20140206.17,
  author = {Karim Christina Scopinho Furquim and Maria Izabel Camargo Mathias and Gislaine Cristina Roma and Letícia Maria Gráballos Ferraz Hebling and Gervásio Henrique Bechara},
  title = {Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days},
  journal = {Animal and Veterinary Sciences},
  volume = {2},
  number = {6},
  pages = {194-207},
  doi = {10.11648/j.avs.20140206.17},
  url = {https://doi.org/10.11648/j.avs.20140206.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140206.17},
  abstract = {A histochemical analysis was performed in this study in order to detect proteins, polysaccharides, lipids, acid phosphatase and calcium in the secretion produced by the salivary glands of Rhipicephalus sanguineus females (Latreille, 1806) (Acari, Ixodidae) fed for 2, 4 and 6 days in New Zealand White rabbits which had been previously immunized with glandular extract obtained from females fed for 4 days (SGE4). The results revealed that such glands presented alterations in their secretory cycle, which occurred:  a) by the inactivity of some c1 cells (in the glands of females fed for 2 days) and c4 (in those fed for 4 days) and b) by the modification in the constitution of the secretion of females fed for 2-6 days. It was verified that, in the glands of females fed for 2 days, there was an increase in proteins and calcium; a reduction in lipids and the contents of polysaccharides and acid phosphatase remained unaltered. In those fed for 4 days there was an increase in proteins, calcium and acid phosphatase; reduction in the lipids, and the content of polysaccharides remained unaltered. In the females fed for 6 days an increase in the components was observed; however, there was a reduction in lipids and acid phosphatase. In addition, it was verified that, in a decrescent order of histochemical alterations, the most affected cells were: f; c2, c3, c5; a, d and c4, e in the glands of females fed for 2 days; c5; a, c2, c3, d and c1, e in the glands of females fed for 4 days and a, c1, c2, d and e, c3 in the glands of females fed for 6 days. The data here obtained clearly show that the most pronounced histochemical modifications were detected in the glands of females fed for 2 and 6 days; however, the modifications observed in the females fed for 4 days must also be considered, once they were significant as well. This has probably occurred because the hosts were inoculated with SGE4, obtained from the salivary glands of females fed for 4 days, intermediate stage of the glandular cycle, which contains antigens that are common to glandular tissues of females fed for 2 and 6 days.},
 year = {2014}
}

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TY  - JOUR
T1  - Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days
AU  - Karim Christina Scopinho Furquim
AU  - Maria Izabel Camargo Mathias
AU  - Gislaine Cristina Roma
AU  - Letícia Maria Gráballos Ferraz Hebling
AU  - Gervásio Henrique Bechara
Y1  - 2014/12/15
PY  - 2014
N1  - https://doi.org/10.11648/j.avs.20140206.17
DO  - 10.11648/j.avs.20140206.17
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 194
EP  - 207
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20140206.17
AB  - A histochemical analysis was performed in this study in order to detect proteins, polysaccharides, lipids, acid phosphatase and calcium in the secretion produced by the salivary glands of Rhipicephalus sanguineus females (Latreille, 1806) (Acari, Ixodidae) fed for 2, 4 and 6 days in New Zealand White rabbits which had been previously immunized with glandular extract obtained from females fed for 4 days (SGE4). The results revealed that such glands presented alterations in their secretory cycle, which occurred:  a) by the inactivity of some c1 cells (in the glands of females fed for 2 days) and c4 (in those fed for 4 days) and b) by the modification in the constitution of the secretion of females fed for 2-6 days. It was verified that, in the glands of females fed for 2 days, there was an increase in proteins and calcium; a reduction in lipids and the contents of polysaccharides and acid phosphatase remained unaltered. In those fed for 4 days there was an increase in proteins, calcium and acid phosphatase; reduction in the lipids, and the content of polysaccharides remained unaltered. In the females fed for 6 days an increase in the components was observed; however, there was a reduction in lipids and acid phosphatase. In addition, it was verified that, in a decrescent order of histochemical alterations, the most affected cells were: f; c2, c3, c5; a, d and c4, e in the glands of females fed for 2 days; c5; a, c2, c3, d and c1, e in the glands of females fed for 4 days and a, c1, c2, d and e, c3 in the glands of females fed for 6 days. The data here obtained clearly show that the most pronounced histochemical modifications were detected in the glands of females fed for 2 and 6 days; however, the modifications observed in the females fed for 4 days must also be considered, once they were significant as well. This has probably occurred because the hosts were inoculated with SGE4, obtained from the salivary glands of females fed for 4 days, intermediate stage of the glandular cycle, which contains antigens that are common to glandular tissues of females fed for 2 and 6 days.
VL  - 2
IS  - 6
ER  -

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Author Information

Karim Christina Scopinho Furquim

Departamento de Biologia, Instituto de Biociências, UNESP, Av. 24 A, no 1515, Cx. Postal 199, CEP: 13506-900, Rio Claro, S.P., Brazil
Maria Izabel Camargo Mathias

Departamento de Biologia, Instituto de Biociências, UNESP, Av. 24 A, no 1515, Cx. Postal 199, CEP: 13506-900, Rio Claro, S.P., Brazil
Gislaine Cristina Roma

Departamento de Biologia, Instituto de Biociências, UNESP, Av. 24 A, no 1515, Cx. Postal 199, CEP: 13506-900, Rio Claro, S.P., Brazil
Letícia Maria Gráballos Ferraz Hebling

Departamento de Biologia, Instituto de Biociências, UNESP, Av. 24 A, no 1515, Cx. Postal 199, CEP: 13506-900, Rio Claro, S.P., Brazil
Gervásio Henrique Bechara

Departamento de Patologia Veterinária, FCAV, UNESP, Via de Acesso Prof. Paulo Castellane, s/n, CEP: 14884-900, Jaboticabal, S.P., Brazil

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Karim Christina Scopinho Furquim, Maria Izabel Camargo Mathias, Gislaine Cristina Roma, Letícia Maria Gráballos Ferraz Hebling, Gervásio Henrique Bechara. (2014). Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days. Animal and Veterinary Sciences, 2(6), 194-207. https://doi.org/10.11648/j.avs.20140206.17

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ACS Style

Karim Christina Scopinho Furquim; Maria Izabel Camargo Mathias; Gislaine Cristina Roma; Letícia Maria Gráballos Ferraz Hebling; Gervásio Henrique Bechara. Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days. Anim. Vet. Sci. 2014, 2(6), 194-207. doi: 10.11648/j.avs.20140206.17

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AMA Style

Karim Christina Scopinho Furquim, Maria Izabel Camargo Mathias, Gislaine Cristina Roma, Letícia Maria Gráballos Ferraz Hebling, Gervásio Henrique Bechara. Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days. Anim Vet Sci. 2014;2(6):194-207. doi: 10.11648/j.avs.20140206.17

Copy | Download

@article{10.11648/j.avs.20140206.17,
  author = {Karim Christina Scopinho Furquim and Maria Izabel Camargo Mathias and Gislaine Cristina Roma and Letícia Maria Gráballos Ferraz Hebling and Gervásio Henrique Bechara},
  title = {Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days},
  journal = {Animal and Veterinary Sciences},
  volume = {2},
  number = {6},
  pages = {194-207},
  doi = {10.11648/j.avs.20140206.17},
  url = {https://doi.org/10.11648/j.avs.20140206.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140206.17},
  abstract = {A histochemical analysis was performed in this study in order to detect proteins, polysaccharides, lipids, acid phosphatase and calcium in the secretion produced by the salivary glands of Rhipicephalus sanguineus females (Latreille, 1806) (Acari, Ixodidae) fed for 2, 4 and 6 days in New Zealand White rabbits which had been previously immunized with glandular extract obtained from females fed for 4 days (SGE4). The results revealed that such glands presented alterations in their secretory cycle, which occurred:  a) by the inactivity of some c1 cells (in the glands of females fed for 2 days) and c4 (in those fed for 4 days) and b) by the modification in the constitution of the secretion of females fed for 2-6 days. It was verified that, in the glands of females fed for 2 days, there was an increase in proteins and calcium; a reduction in lipids and the contents of polysaccharides and acid phosphatase remained unaltered. In those fed for 4 days there was an increase in proteins, calcium and acid phosphatase; reduction in the lipids, and the content of polysaccharides remained unaltered. In the females fed for 6 days an increase in the components was observed; however, there was a reduction in lipids and acid phosphatase. In addition, it was verified that, in a decrescent order of histochemical alterations, the most affected cells were: f; c2, c3, c5; a, d and c4, e in the glands of females fed for 2 days; c5; a, c2, c3, d and c1, e in the glands of females fed for 4 days and a, c1, c2, d and e, c3 in the glands of females fed for 6 days. The data here obtained clearly show that the most pronounced histochemical modifications were detected in the glands of females fed for 2 and 6 days; however, the modifications observed in the females fed for 4 days must also be considered, once they were significant as well. This has probably occurred because the hosts were inoculated with SGE4, obtained from the salivary glands of females fed for 4 days, intermediate stage of the glandular cycle, which contains antigens that are common to glandular tissues of females fed for 2 and 6 days.},
 year = {2014}
}

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TY  - JOUR
T1  - Morphological Plasticity of Ticks’ Salivary Glands and the Meaning of Hematophagy in Hosts Immunized with Glandular Extract of Females Fed for 4 Days
AU  - Karim Christina Scopinho Furquim
AU  - Maria Izabel Camargo Mathias
AU  - Gislaine Cristina Roma
AU  - Letícia Maria Gráballos Ferraz Hebling
AU  - Gervásio Henrique Bechara
Y1  - 2014/12/15
PY  - 2014
N1  - https://doi.org/10.11648/j.avs.20140206.17
DO  - 10.11648/j.avs.20140206.17
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 194
EP  - 207
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20140206.17
AB  - A histochemical analysis was performed in this study in order to detect proteins, polysaccharides, lipids, acid phosphatase and calcium in the secretion produced by the salivary glands of Rhipicephalus sanguineus females (Latreille, 1806) (Acari, Ixodidae) fed for 2, 4 and 6 days in New Zealand White rabbits which had been previously immunized with glandular extract obtained from females fed for 4 days (SGE4). The results revealed that such glands presented alterations in their secretory cycle, which occurred:  a) by the inactivity of some c1 cells (in the glands of females fed for 2 days) and c4 (in those fed for 4 days) and b) by the modification in the constitution of the secretion of females fed for 2-6 days. It was verified that, in the glands of females fed for 2 days, there was an increase in proteins and calcium; a reduction in lipids and the contents of polysaccharides and acid phosphatase remained unaltered. In those fed for 4 days there was an increase in proteins, calcium and acid phosphatase; reduction in the lipids, and the content of polysaccharides remained unaltered. In the females fed for 6 days an increase in the components was observed; however, there was a reduction in lipids and acid phosphatase. In addition, it was verified that, in a decrescent order of histochemical alterations, the most affected cells were: f; c2, c3, c5; a, d and c4, e in the glands of females fed for 2 days; c5; a, c2, c3, d and c1, e in the glands of females fed for 4 days and a, c1, c2, d and e, c3 in the glands of females fed for 6 days. The data here obtained clearly show that the most pronounced histochemical modifications were detected in the glands of females fed for 2 and 6 days; however, the modifications observed in the females fed for 4 days must also be considered, once they were significant as well. This has probably occurred because the hosts were inoculated with SGE4, obtained from the salivary glands of females fed for 4 days, intermediate stage of the glandular cycle, which contains antigens that are common to glandular tissues of females fed for 2 and 6 days.
VL  - 2
IS  - 6
ER  -

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