Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo

Zulifqar Ali Laghari; Ayaz Ali Samo; Baradi Waryani; Zameer Ali Palh; Khalid Hussain Lashari; Ghulam Murtaza Mastoi; Gulshan Ara Sahato; Tahira Jabeen Ursani

doi:doi:10.11648/j.avs.20150301.12

Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo

Zulifqar Ali Laghari, Ayaz Ali Samo, Baradi Waryani, Zameer Ali Palh, Khalid Hussain Lashari, Ghulam Murtaza Mastoi, Gulshan Ara Sahato, Tahira Jabeen Ursani

Published in Animal and Veterinary Sciences (Volume 3, Issue 1)

Received: 5 September 2014 Accepted: 3 December 2014 Published: 20 January 2015

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Abstract

The developing Chick has been widely used as a model organism for research studies in developmental biology. Various aspects of ethanol (EtOH) toxicity during embryonic development have been documented in experimental studies. The studies on chick embryo address the effects of EtOH on craniofacial abnormalities and angiogenesis. The purpose of this study was to assess the effects of a single dose of ethanol on survival rate and angiogenesis of chick embryos during early stages of development. Chick eggs were incubated at 37ºC in the humidified incubator, after 72 hours of incubation these eggs were removed from incubator, and treated with a single doses of 1%, 5%, 10%, 13% and 15% EtOH. The effects on survival rate and angiogenesis were recorded on ethanol treated chick embryos. The survival rate was slightly reduced with 1% and 5% EtOH, however with increasing dose of 10% the survival rate was reduced to 64% and at 15% EtOH complete death was observed. Our results also indicate that treatment of EtOH with 1% and 5% did not have any obvious effects on vessels formation in comparison with the BSS treated embryos. However, treatment of chick embryo with 10%, 13% and 15% EtOH severely inhibited the blood vessels formation.

Published in	Animal and Veterinary Sciences (Volume 3, Issue 1)
DOI	10.11648/j.avs.20150301.12
Page(s)	8-11
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Chick Embryo, Angiogenesis, EtOH

References

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APA Style

Zulifqar Ali Laghari, Ayaz Ali Samo, Baradi Waryani, Zameer Ali Palh, Khalid Hussain Lashari, et al. (2015). Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo. Animal and Veterinary Sciences, 3(1), 8-11. https://doi.org/10.11648/j.avs.20150301.12

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ACS Style

Zulifqar Ali Laghari; Ayaz Ali Samo; Baradi Waryani; Zameer Ali Palh; Khalid Hussain Lashari, et al. Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo. Anim. Vet. Sci. 2015, 3(1), 8-11. doi: 10.11648/j.avs.20150301.12

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AMA Style

Zulifqar Ali Laghari, Ayaz Ali Samo, Baradi Waryani, Zameer Ali Palh, Khalid Hussain Lashari, et al. Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo. Anim Vet Sci. 2015;3(1):8-11. doi: 10.11648/j.avs.20150301.12

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@article{10.11648/j.avs.20150301.12,
  author = {Zulifqar Ali Laghari and Ayaz Ali Samo and Baradi Waryani and Zameer Ali Palh and Khalid Hussain Lashari and Ghulam Murtaza Mastoi and Gulshan Ara Sahato and Tahira Jabeen Ursani},
  title = {Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo},
  journal = {Animal and Veterinary Sciences},
  volume = {3},
  number = {1},
  pages = {8-11},
  doi = {10.11648/j.avs.20150301.12},
  url = {https://doi.org/10.11648/j.avs.20150301.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150301.12},
  abstract = {The developing Chick has been widely used as a model organism for research studies in developmental biology. Various aspects of ethanol (EtOH) toxicity during embryonic development have been documented in experimental studies. The studies on chick embryo address the effects of EtOH on craniofacial abnormalities and angiogenesis. The purpose of this study was to assess the effects of a single dose of ethanol on survival rate and angiogenesis of chick embryos during early stages of development. Chick eggs were incubated at 37ºC in the humidified incubator, after 72 hours of incubation  these eggs were removed from incubator, and treated with a single doses of 1%, 5%, 10%, 13% and 15% EtOH. The effects on survival rate and angiogenesis were recorded on ethanol treated chick embryos. The survival rate was slightly reduced with 1% and 5% EtOH, however with increasing dose of 10% the survival rate was reduced to 64% and at 15% EtOH complete death was observed. Our results also indicate that treatment of EtOH with 1% and 5% did not have any obvious effects on vessels formation in comparison with the BSS treated embryos. However, treatment of chick embryo with 10%, 13% and 15% EtOH severely inhibited the blood vessels formation.},
 year = {2015}
}

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TY  - JOUR
T1  - Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo
AU  - Zulifqar Ali Laghari
AU  - Ayaz Ali Samo
AU  - Baradi Waryani
AU  - Zameer Ali Palh
AU  - Khalid Hussain Lashari
AU  - Ghulam Murtaza Mastoi
AU  - Gulshan Ara Sahato
AU  - Tahira Jabeen Ursani
Y1  - 2015/01/20
PY  - 2015
N1  - https://doi.org/10.11648/j.avs.20150301.12
DO  - 10.11648/j.avs.20150301.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 8
EP  - 11
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20150301.12
AB  - The developing Chick has been widely used as a model organism for research studies in developmental biology. Various aspects of ethanol (EtOH) toxicity during embryonic development have been documented in experimental studies. The studies on chick embryo address the effects of EtOH on craniofacial abnormalities and angiogenesis. The purpose of this study was to assess the effects of a single dose of ethanol on survival rate and angiogenesis of chick embryos during early stages of development. Chick eggs were incubated at 37ºC in the humidified incubator, after 72 hours of incubation  these eggs were removed from incubator, and treated with a single doses of 1%, 5%, 10%, 13% and 15% EtOH. The effects on survival rate and angiogenesis were recorded on ethanol treated chick embryos. The survival rate was slightly reduced with 1% and 5% EtOH, however with increasing dose of 10% the survival rate was reduced to 64% and at 15% EtOH complete death was observed. Our results also indicate that treatment of EtOH with 1% and 5% did not have any obvious effects on vessels formation in comparison with the BSS treated embryos. However, treatment of chick embryo with 10%, 13% and 15% EtOH severely inhibited the blood vessels formation.
VL  - 3
IS  - 1
ER  -

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Author Information

Zulifqar Ali Laghari

Department of Physiology, University of Sindh, Jamshoro, Sindh, Pakistan
Ayaz Ali Samo

Department of Physiology, University of Sindh, Jamshoro, Sindh, Pakistan
Baradi Waryani

Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan
Zameer Ali Palh

Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan
Khalid Hussain Lashari

Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan
Ghulam Murtaza Mastoi

Centre for Environmental Sciences, University of Sindh, Jamshoro, Sindh, Pakistan
Gulshan Ara Sahato

Department of Fresh Water Biology and fisheries, University of Sindh, Jamshoro, Sindh, Pakistan
Tahira Jabeen Ursani

Department of Zoology, University of Sindh, Jamshoro, Sindh, Pakistan

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Zulifqar Ali Laghari, Ayaz Ali Samo, Baradi Waryani, Zameer Ali Palh, Khalid Hussain Lashari, et al. (2015). Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo. Animal and Veterinary Sciences, 3(1), 8-11. https://doi.org/10.11648/j.avs.20150301.12

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ACS Style

Zulifqar Ali Laghari; Ayaz Ali Samo; Baradi Waryani; Zameer Ali Palh; Khalid Hussain Lashari, et al. Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo. Anim. Vet. Sci. 2015, 3(1), 8-11. doi: 10.11648/j.avs.20150301.12

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AMA Style

Zulifqar Ali Laghari, Ayaz Ali Samo, Baradi Waryani, Zameer Ali Palh, Khalid Hussain Lashari, et al. Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo. Anim Vet Sci. 2015;3(1):8-11. doi: 10.11648/j.avs.20150301.12

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@article{10.11648/j.avs.20150301.12,
  author = {Zulifqar Ali Laghari and Ayaz Ali Samo and Baradi Waryani and Zameer Ali Palh and Khalid Hussain Lashari and Ghulam Murtaza Mastoi and Gulshan Ara Sahato and Tahira Jabeen Ursani},
  title = {Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo},
  journal = {Animal and Veterinary Sciences},
  volume = {3},
  number = {1},
  pages = {8-11},
  doi = {10.11648/j.avs.20150301.12},
  url = {https://doi.org/10.11648/j.avs.20150301.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150301.12},
  abstract = {The developing Chick has been widely used as a model organism for research studies in developmental biology. Various aspects of ethanol (EtOH) toxicity during embryonic development have been documented in experimental studies. The studies on chick embryo address the effects of EtOH on craniofacial abnormalities and angiogenesis. The purpose of this study was to assess the effects of a single dose of ethanol on survival rate and angiogenesis of chick embryos during early stages of development. Chick eggs were incubated at 37ºC in the humidified incubator, after 72 hours of incubation  these eggs were removed from incubator, and treated with a single doses of 1%, 5%, 10%, 13% and 15% EtOH. The effects on survival rate and angiogenesis were recorded on ethanol treated chick embryos. The survival rate was slightly reduced with 1% and 5% EtOH, however with increasing dose of 10% the survival rate was reduced to 64% and at 15% EtOH complete death was observed. Our results also indicate that treatment of EtOH with 1% and 5% did not have any obvious effects on vessels formation in comparison with the BSS treated embryos. However, treatment of chick embryo with 10%, 13% and 15% EtOH severely inhibited the blood vessels formation.},
 year = {2015}
}

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TY  - JOUR
T1  - Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo
AU  - Zulifqar Ali Laghari
AU  - Ayaz Ali Samo
AU  - Baradi Waryani
AU  - Zameer Ali Palh
AU  - Khalid Hussain Lashari
AU  - Ghulam Murtaza Mastoi
AU  - Gulshan Ara Sahato
AU  - Tahira Jabeen Ursani
Y1  - 2015/01/20
PY  - 2015
N1  - https://doi.org/10.11648/j.avs.20150301.12
DO  - 10.11648/j.avs.20150301.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 8
EP  - 11
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20150301.12
AB  - The developing Chick has been widely used as a model organism for research studies in developmental biology. Various aspects of ethanol (EtOH) toxicity during embryonic development have been documented in experimental studies. The studies on chick embryo address the effects of EtOH on craniofacial abnormalities and angiogenesis. The purpose of this study was to assess the effects of a single dose of ethanol on survival rate and angiogenesis of chick embryos during early stages of development. Chick eggs were incubated at 37ºC in the humidified incubator, after 72 hours of incubation  these eggs were removed from incubator, and treated with a single doses of 1%, 5%, 10%, 13% and 15% EtOH. The effects on survival rate and angiogenesis were recorded on ethanol treated chick embryos. The survival rate was slightly reduced with 1% and 5% EtOH, however with increasing dose of 10% the survival rate was reduced to 64% and at 15% EtOH complete death was observed. Our results also indicate that treatment of EtOH with 1% and 5% did not have any obvious effects on vessels formation in comparison with the BSS treated embryos. However, treatment of chick embryo with 10%, 13% and 15% EtOH severely inhibited the blood vessels formation.
VL  - 3
IS  - 1
ER  -

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