HLA Alloantibodies in Multiparous Women at Abidjan

N’guessan Koffi; Akre Dagra Paul; Dasse Sery Romuald; Gobey Richard Arthur; Fizet Dominique; Seka Seka Joseph; Sombo Mambo François

doi:doi:10.11648/j.iji.20160406.13

HLA Alloantibodies in Multiparous Women at Abidjan

N’guessan Koffi, Akre Dagra Paul, Dasse Sery Romuald, Gobey Richard Arthur, Fizet Dominique, Seka Seka Joseph, Sombo Mambo François

Published in International Journal of Immunology (Volume 4, Issue 6)

Received: 4 October 2016 Accepted: 23 November 2016 Published: 29 December 2016

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Abstract

Serum of multiparous women can contain HLA polyclonal antibodies which can be used as reagents for serological typing of HLA class I and class II. The aim of this study was to investigate an alloimmunization of multiparous women and to identify HLA polyclonal antibodies which could be useful as laboratory reagents in view of the future HLA unit of the Department of Immunology at Abidjan. In an experimental and analytic study, we screened HLA antibodies using microlymphocytotoxicity test on sera of 121 multiparous women aged from 18 to 70 years old, in apparent good health, transfused or no-transfused and with at least two parities. 37.19% of the multiparous women were alloimmunized. 13 women were sensitized to HLA class I, 18 to HLA class II and 14 were both sensitized to class I and class II. Specificity of the screened antibodies was identified at 17.78%. HLA-B7 specificity (Class I) was 37.5% and class II specificity (HLA-DR4, DR7, DR13, DR13+14, DRw52) was 62.5%. Specificity was evaluated by the correlation coefficient. For class I specificity correlation coefficients were ranged from 0.79 to 1, while for class II specificities, correlation coefficients were ranged from 0.54 to 0.9. Screening of HLA antibodies on multiparous women at Abidjan was positive and specificities were determined. This enabled authors to the possibility of using multiparous women sera as a source of production of polyclonal antibodies for serological HLA typing in the HLA unit of the Department of Immunology at Abidjan.

Published in	International Journal of Immunology (Volume 4, Issue 6)
DOI	10.11648/j.iji.20160406.13
Page(s)	64-67
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

HLA Alloimmunization, Multiparous Women, Microlymphocytotoxicity, Ivory Coast

References

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[10]	Tomicić M, Starcević M, Bux J, Zach V, Hundrić-Haspl Z, Drazić V, Grahovac B. Severe neonatal neutropenia due to anti-human leucocyte antigen B49 alloimmunization only: a case report. Transfus Med. 2003; 13 (4): 233-7.
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[15]	Morin-Papunel L, Tiilikainen A, Hartkainen-Sorri AL. Maternal HLA immunization during pregnancy: presence of anti HLA antibodies in half of multigravidous women. Med Biol. 1984; 62 (6): 323-5.
[16]	Colombani, J. "HLA: Fonctions immunitaires et applications médicales" John Libbey Eurotext. Paris 1993, pp 285.
[17]	Houissa B, Abdelkefi S, Bouslama M, Naggara M, Zaier M, Hmida S, Yacoub S. The utility of class I HLA antibodies screening in Tunisian obstetric departement in order to select good HLA typing reagents. Ann Biol Clin. 2003; 61 (4): 435-9.
[18]	Rafoux C, Lepage V.-Polymorphisme sérologique de classe I. La série allélique A, B, C.-in HLA. Complexe Majeur d’Histocompatibilité de l’Homme, Jean Dausset, Marika PL. Flammarion Medecine Sciences. Paris 1989, pp 64-100.
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APA Style

N’guessan Koffi, Akre Dagra Paul, Dasse Sery Romuald, Gobey Richard Arthur, Fizet Dominique, et al. (2016). HLA Alloantibodies in Multiparous Women at Abidjan. International Journal of Immunology, 4(6), 64-67. https://doi.org/10.11648/j.iji.20160406.13

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ACS Style

N’guessan Koffi; Akre Dagra Paul; Dasse Sery Romuald; Gobey Richard Arthur; Fizet Dominique, et al. HLA Alloantibodies in Multiparous Women at Abidjan. Int. J. Immunol. 2016, 4(6), 64-67. doi: 10.11648/j.iji.20160406.13

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AMA Style

N’guessan Koffi, Akre Dagra Paul, Dasse Sery Romuald, Gobey Richard Arthur, Fizet Dominique, et al. HLA Alloantibodies in Multiparous Women at Abidjan. Int J Immunol. 2016;4(6):64-67. doi: 10.11648/j.iji.20160406.13

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@article{10.11648/j.iji.20160406.13,
  author = {N’guessan Koffi and Akre Dagra Paul and Dasse Sery Romuald and Gobey Richard Arthur and Fizet Dominique and Seka Seka Joseph and Sombo Mambo François},
  title = {HLA Alloantibodies in Multiparous Women at Abidjan},
  journal = {International Journal of Immunology},
  volume = {4},
  number = {6},
  pages = {64-67},
  doi = {10.11648/j.iji.20160406.13},
  url = {https://doi.org/10.11648/j.iji.20160406.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20160406.13},
  abstract = {Serum of multiparous women can contain HLA polyclonal antibodies which can be used as reagents for serological typing of HLA class I and class II. The aim of this study was to investigate an alloimmunization of multiparous women and to identify HLA polyclonal antibodies which could be useful as laboratory reagents in view of the future HLA unit of the Department of Immunology at Abidjan. In an experimental and analytic study, we screened HLA antibodies using microlymphocytotoxicity test on sera of 121 multiparous women aged from 18 to 70 years old, in apparent good health, transfused or no-transfused and with at least two parities. 37.19% of the multiparous women were alloimmunized. 13 women were sensitized to HLA class I, 18 to HLA class II and 14 were both sensitized to class I and class II. Specificity of the screened antibodies was identified at 17.78%. HLA-B7 specificity (Class I) was 37.5% and class II specificity (HLA-DR4, DR7, DR13, DR13+14, DRw52) was 62.5%. Specificity was evaluated by the correlation coefficient. For class I specificity correlation coefficients were ranged from 0.79 to 1, while for class II specificities, correlation coefficients were ranged from 0.54 to 0.9. Screening of HLA antibodies on multiparous women at Abidjan was positive and specificities were determined. This enabled authors to the possibility of using multiparous women sera as a source of production of polyclonal antibodies for serological HLA typing in the HLA unit of the Department of Immunology at Abidjan.},
 year = {2016}
}

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TY  - JOUR
T1  - HLA Alloantibodies in Multiparous Women at Abidjan
AU  - N’guessan Koffi
AU  - Akre Dagra Paul
AU  - Dasse Sery Romuald
AU  - Gobey Richard Arthur
AU  - Fizet Dominique
AU  - Seka Seka Joseph
AU  - Sombo Mambo François
Y1  - 2016/12/29
PY  - 2016
N1  - https://doi.org/10.11648/j.iji.20160406.13
DO  - 10.11648/j.iji.20160406.13
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 64
EP  - 67
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20160406.13
AB  - Serum of multiparous women can contain HLA polyclonal antibodies which can be used as reagents for serological typing of HLA class I and class II. The aim of this study was to investigate an alloimmunization of multiparous women and to identify HLA polyclonal antibodies which could be useful as laboratory reagents in view of the future HLA unit of the Department of Immunology at Abidjan. In an experimental and analytic study, we screened HLA antibodies using microlymphocytotoxicity test on sera of 121 multiparous women aged from 18 to 70 years old, in apparent good health, transfused or no-transfused and with at least two parities. 37.19% of the multiparous women were alloimmunized. 13 women were sensitized to HLA class I, 18 to HLA class II and 14 were both sensitized to class I and class II. Specificity of the screened antibodies was identified at 17.78%. HLA-B7 specificity (Class I) was 37.5% and class II specificity (HLA-DR4, DR7, DR13, DR13+14, DRw52) was 62.5%. Specificity was evaluated by the correlation coefficient. For class I specificity correlation coefficients were ranged from 0.79 to 1, while for class II specificities, correlation coefficients were ranged from 0.54 to 0.9. Screening of HLA antibodies on multiparous women at Abidjan was positive and specificities were determined. This enabled authors to the possibility of using multiparous women sera as a source of production of polyclonal antibodies for serological HLA typing in the HLA unit of the Department of Immunology at Abidjan.
VL  - 4
IS  - 6
ER  -

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Author Information

N’guessan Koffi

Department of Immunology-Hematology and Cancerology, Felix Houphou?t Boigny University, Abidjan, Ivory Coast
Akre Dagra Paul

Department of Immunology, Allassane Ouattara University, Bouake, Ivory Coast
Dasse Sery Romuald

Department of Immunology-Hematology and Cancerology, Felix Houphou?t Boigny University, Abidjan, Ivory Coast
Gobey Richard Arthur

Department of Immunology, Allassane Ouattara University, Bouake, Ivory Coast
Fizet Dominique

Department of Cellular Immunology, French Blood Agency, Bordeaux, France
Seka Seka Joseph

Department of Immunology, Allassane Ouattara University, Bouake, Ivory Coast
Sombo Mambo François

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Plain Text BibTeX RIS

APA Style

N’guessan Koffi, Akre Dagra Paul, Dasse Sery Romuald, Gobey Richard Arthur, Fizet Dominique, et al. (2016). HLA Alloantibodies in Multiparous Women at Abidjan. International Journal of Immunology, 4(6), 64-67. https://doi.org/10.11648/j.iji.20160406.13

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ACS Style

N’guessan Koffi; Akre Dagra Paul; Dasse Sery Romuald; Gobey Richard Arthur; Fizet Dominique, et al. HLA Alloantibodies in Multiparous Women at Abidjan. Int. J. Immunol. 2016, 4(6), 64-67. doi: 10.11648/j.iji.20160406.13

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AMA Style

N’guessan Koffi, Akre Dagra Paul, Dasse Sery Romuald, Gobey Richard Arthur, Fizet Dominique, et al. HLA Alloantibodies in Multiparous Women at Abidjan. Int J Immunol. 2016;4(6):64-67. doi: 10.11648/j.iji.20160406.13

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@article{10.11648/j.iji.20160406.13,
  author = {N’guessan Koffi and Akre Dagra Paul and Dasse Sery Romuald and Gobey Richard Arthur and Fizet Dominique and Seka Seka Joseph and Sombo Mambo François},
  title = {HLA Alloantibodies in Multiparous Women at Abidjan},
  journal = {International Journal of Immunology},
  volume = {4},
  number = {6},
  pages = {64-67},
  doi = {10.11648/j.iji.20160406.13},
  url = {https://doi.org/10.11648/j.iji.20160406.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20160406.13},
  abstract = {Serum of multiparous women can contain HLA polyclonal antibodies which can be used as reagents for serological typing of HLA class I and class II. The aim of this study was to investigate an alloimmunization of multiparous women and to identify HLA polyclonal antibodies which could be useful as laboratory reagents in view of the future HLA unit of the Department of Immunology at Abidjan. In an experimental and analytic study, we screened HLA antibodies using microlymphocytotoxicity test on sera of 121 multiparous women aged from 18 to 70 years old, in apparent good health, transfused or no-transfused and with at least two parities. 37.19% of the multiparous women were alloimmunized. 13 women were sensitized to HLA class I, 18 to HLA class II and 14 were both sensitized to class I and class II. Specificity of the screened antibodies was identified at 17.78%. HLA-B7 specificity (Class I) was 37.5% and class II specificity (HLA-DR4, DR7, DR13, DR13+14, DRw52) was 62.5%. Specificity was evaluated by the correlation coefficient. For class I specificity correlation coefficients were ranged from 0.79 to 1, while for class II specificities, correlation coefficients were ranged from 0.54 to 0.9. Screening of HLA antibodies on multiparous women at Abidjan was positive and specificities were determined. This enabled authors to the possibility of using multiparous women sera as a source of production of polyclonal antibodies for serological HLA typing in the HLA unit of the Department of Immunology at Abidjan.},
 year = {2016}
}

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TY  - JOUR
T1  - HLA Alloantibodies in Multiparous Women at Abidjan
AU  - N’guessan Koffi
AU  - Akre Dagra Paul
AU  - Dasse Sery Romuald
AU  - Gobey Richard Arthur
AU  - Fizet Dominique
AU  - Seka Seka Joseph
AU  - Sombo Mambo François
Y1  - 2016/12/29
PY  - 2016
N1  - https://doi.org/10.11648/j.iji.20160406.13
DO  - 10.11648/j.iji.20160406.13
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 64
EP  - 67
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20160406.13
AB  - Serum of multiparous women can contain HLA polyclonal antibodies which can be used as reagents for serological typing of HLA class I and class II. The aim of this study was to investigate an alloimmunization of multiparous women and to identify HLA polyclonal antibodies which could be useful as laboratory reagents in view of the future HLA unit of the Department of Immunology at Abidjan. In an experimental and analytic study, we screened HLA antibodies using microlymphocytotoxicity test on sera of 121 multiparous women aged from 18 to 70 years old, in apparent good health, transfused or no-transfused and with at least two parities. 37.19% of the multiparous women were alloimmunized. 13 women were sensitized to HLA class I, 18 to HLA class II and 14 were both sensitized to class I and class II. Specificity of the screened antibodies was identified at 17.78%. HLA-B7 specificity (Class I) was 37.5% and class II specificity (HLA-DR4, DR7, DR13, DR13+14, DRw52) was 62.5%. Specificity was evaluated by the correlation coefficient. For class I specificity correlation coefficients were ranged from 0.79 to 1, while for class II specificities, correlation coefficients were ranged from 0.54 to 0.9. Screening of HLA antibodies on multiparous women at Abidjan was positive and specificities were determined. This enabled authors to the possibility of using multiparous women sera as a source of production of polyclonal antibodies for serological HLA typing in the HLA unit of the Department of Immunology at Abidjan.
VL  - 4
IS  - 6
ER  -

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