Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19)

Fernández Angulo Daniela; Lewis Luján Lidianys María; Iloki Assanga Simon Bernard; Gil-Salido Armida Andrea; Lara Espinoza Claudia Lizeth; Rubio-Pino José Luis

doi:doi:10.11648/j.ajbio.20150303.15

Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19)

Fernández Angulo Daniela, Lewis Luján Lidianys María, Iloki Assanga Simon Bernard, Gil-Salido Armida Andrea, Lara Espinoza Claudia Lizeth, Rubio-Pino José Luis

Published in American Journal of BioScience (Volume 3, Issue 3)

Received: 7 April 2015 Accepted: 18 April 2015 Published: 13 May 2015

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Abstract

Hydrogen peroxide is a normal by-product of cellular metabolism that in higher concentrations can cause oxidative stress. Reactive oxygen species impair the physiological functions of retinal pigment epithelial (RPE) cells, which are known as one major cause of ocular pathologies. Most studies investigating the influence of H2O2 on cells in culture but H2O2 concentrations are not sustained in culture medium. Continuous generation using glucose oxidase (GOx) system allows application of relevant low H2O2 concentrations over physiologically relevant times periods (up to 24 h). Recent findings suggest that bolus and GOx treatments can lead to different cellular response, thus warranting a quantitative comparison between the two approaches. When added as a pulse H2O2 is rapidly depleted. Continuous generation of H2O2 produces different behavior in function of GOx activities. Cytotoxicity analyses show that cells can tolerate short exposure to high H2O2 doses delivered as a pulse but are susceptible to lower continuous doses. Application of hydrogen peroxide causes a concentration-dependent decrease in the intracellular glutathione (GSH) content that was accompanied by a matching decrease in the glutathione peroxide activity and reducing power (FRAP).

Published in	American Journal of BioScience (Volume 3, Issue 3)
DOI	10.11648/j.ajbio.20150303.15
Page(s)	93-113
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Hydrogen Peroxide, Glucose Oxidase, Arpe-19 Cells, Cellular Redox Status, Free Radicals

References

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APA Style

Fernández Angulo Daniela, Lewis Luján Lidianys María, Iloki Assanga Simon Bernard, Gil-Salido Armida Andrea, Lara Espinoza Claudia Lizeth, et al. (2015). Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19). American Journal of BioScience, 3(3), 93-113. https://doi.org/10.11648/j.ajbio.20150303.15

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ACS Style

Fernández Angulo Daniela; Lewis Luján Lidianys María; Iloki Assanga Simon Bernard; Gil-Salido Armida Andrea; Lara Espinoza Claudia Lizeth, et al. Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19). Am. J. BioScience 2015, 3(3), 93-113. doi: 10.11648/j.ajbio.20150303.15

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AMA Style

Fernández Angulo Daniela, Lewis Luján Lidianys María, Iloki Assanga Simon Bernard, Gil-Salido Armida Andrea, Lara Espinoza Claudia Lizeth, et al. Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19). Am J BioScience. 2015;3(3):93-113. doi: 10.11648/j.ajbio.20150303.15

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@article{10.11648/j.ajbio.20150303.15,
  author = {Fernández Angulo Daniela and Lewis Luján Lidianys María and Iloki Assanga Simon Bernard and Gil-Salido Armida Andrea and Lara Espinoza Claudia Lizeth and Rubio-Pino José Luis},
  title = {Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19)},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {3},
  pages = {93-113},
  doi = {10.11648/j.ajbio.20150303.15},
  url = {https://doi.org/10.11648/j.ajbio.20150303.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150303.15},
  abstract = {Hydrogen peroxide is a normal by-product of cellular metabolism that in higher concentrations can cause oxidative stress. Reactive oxygen species impair the physiological functions of retinal pigment epithelial (RPE) cells, which are known as one major cause of ocular pathologies. Most studies investigating the influence of H2O2 on cells in culture but H2O2 concentrations are not sustained in culture medium. Continuous generation using glucose oxidase (GOx) system allows application of relevant low H2O2 concentrations over physiologically relevant times periods (up to 24 h). Recent findings suggest that bolus and GOx treatments can lead to different cellular response, thus warranting a quantitative comparison between the two approaches. When added as a pulse H2O2 is rapidly depleted. Continuous generation of H2O2 produces different behavior in function of GOx activities. Cytotoxicity analyses show that cells can tolerate short exposure to high H2O2 doses delivered as a pulse but are susceptible to lower continuous doses. Application of hydrogen peroxide causes a concentration-dependent decrease in the intracellular glutathione (GSH) content that was accompanied by a matching decrease in the glutathione peroxide activity and reducing power (FRAP).},
 year = {2015}
}

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TY  - JOUR
T1  - Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19)
AU  - Fernández Angulo Daniela
AU  - Lewis Luján Lidianys María
AU  - Iloki Assanga Simon Bernard
AU  - Gil-Salido Armida Andrea
AU  - Lara Espinoza Claudia Lizeth
AU  - Rubio-Pino José Luis
Y1  - 2015/05/13
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbio.20150303.15
DO  - 10.11648/j.ajbio.20150303.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 93
EP  - 113
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20150303.15
AB  - Hydrogen peroxide is a normal by-product of cellular metabolism that in higher concentrations can cause oxidative stress. Reactive oxygen species impair the physiological functions of retinal pigment epithelial (RPE) cells, which are known as one major cause of ocular pathologies. Most studies investigating the influence of H2O2 on cells in culture but H2O2 concentrations are not sustained in culture medium. Continuous generation using glucose oxidase (GOx) system allows application of relevant low H2O2 concentrations over physiologically relevant times periods (up to 24 h). Recent findings suggest that bolus and GOx treatments can lead to different cellular response, thus warranting a quantitative comparison between the two approaches. When added as a pulse H2O2 is rapidly depleted. Continuous generation of H2O2 produces different behavior in function of GOx activities. Cytotoxicity analyses show that cells can tolerate short exposure to high H2O2 doses delivered as a pulse but are susceptible to lower continuous doses. Application of hydrogen peroxide causes a concentration-dependent decrease in the intracellular glutathione (GSH) content that was accompanied by a matching decrease in the glutathione peroxide activity and reducing power (FRAP).
VL  - 3
IS  - 3
ER  -

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Author Information

Fernández Angulo Daniela

Department of LIBAF, Rubio Pharma y Asociados S.A. de C.V. Blvd. El Llano. Hermosillo, Sonora, Mexico
Lewis Luján Lidianys María

Department of LIBAF, Rubio Pharma y Asociados S.A. de C.V. Blvd. El Llano. Hermosillo, Sonora, Mexico
Iloki Assanga Simon Bernard

Department of LIBAF, Rubio Pharma y Asociados S.A. de C.V. Blvd. El Llano. Hermosillo, Sonora, Mexico
Gil-Salido Armida Andrea

Department of LIBAF, Rubio Pharma y Asociados S.A. de C.V. Blvd. El Llano. Hermosillo, Sonora, Mexico
Lara Espinoza Claudia Lizeth

Department of LIBAF, Rubio Pharma y Asociados S.A. de C.V. Blvd. El Llano. Hermosillo, Sonora, Mexico
Rubio-Pino José Luis

Department of LIBAF, Rubio Pharma y Asociados S.A. de C.V. Blvd. El Llano. Hermosillo, Sonora, Mexico

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Fernández Angulo Daniela, Lewis Luján Lidianys María, Iloki Assanga Simon Bernard, Gil-Salido Armida Andrea, Lara Espinoza Claudia Lizeth, et al. (2015). Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19). American Journal of BioScience, 3(3), 93-113. https://doi.org/10.11648/j.ajbio.20150303.15

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ACS Style

Fernández Angulo Daniela; Lewis Luján Lidianys María; Iloki Assanga Simon Bernard; Gil-Salido Armida Andrea; Lara Espinoza Claudia Lizeth, et al. Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19). Am. J. BioScience 2015, 3(3), 93-113. doi: 10.11648/j.ajbio.20150303.15

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AMA Style

Fernández Angulo Daniela, Lewis Luján Lidianys María, Iloki Assanga Simon Bernard, Gil-Salido Armida Andrea, Lara Espinoza Claudia Lizeth, et al. Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19). Am J BioScience. 2015;3(3):93-113. doi: 10.11648/j.ajbio.20150303.15

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@article{10.11648/j.ajbio.20150303.15,
  author = {Fernández Angulo Daniela and Lewis Luján Lidianys María and Iloki Assanga Simon Bernard and Gil-Salido Armida Andrea and Lara Espinoza Claudia Lizeth and Rubio-Pino José Luis},
  title = {Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19)},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {3},
  pages = {93-113},
  doi = {10.11648/j.ajbio.20150303.15},
  url = {https://doi.org/10.11648/j.ajbio.20150303.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150303.15},
  abstract = {Hydrogen peroxide is a normal by-product of cellular metabolism that in higher concentrations can cause oxidative stress. Reactive oxygen species impair the physiological functions of retinal pigment epithelial (RPE) cells, which are known as one major cause of ocular pathologies. Most studies investigating the influence of H2O2 on cells in culture but H2O2 concentrations are not sustained in culture medium. Continuous generation using glucose oxidase (GOx) system allows application of relevant low H2O2 concentrations over physiologically relevant times periods (up to 24 h). Recent findings suggest that bolus and GOx treatments can lead to different cellular response, thus warranting a quantitative comparison between the two approaches. When added as a pulse H2O2 is rapidly depleted. Continuous generation of H2O2 produces different behavior in function of GOx activities. Cytotoxicity analyses show that cells can tolerate short exposure to high H2O2 doses delivered as a pulse but are susceptible to lower continuous doses. Application of hydrogen peroxide causes a concentration-dependent decrease in the intracellular glutathione (GSH) content that was accompanied by a matching decrease in the glutathione peroxide activity and reducing power (FRAP).},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19)
AU  - Fernández Angulo Daniela
AU  - Lewis Luján Lidianys María
AU  - Iloki Assanga Simon Bernard
AU  - Gil-Salido Armida Andrea
AU  - Lara Espinoza Claudia Lizeth
AU  - Rubio-Pino José Luis
Y1  - 2015/05/13
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbio.20150303.15
DO  - 10.11648/j.ajbio.20150303.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 93
EP  - 113
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20150303.15
AB  - Hydrogen peroxide is a normal by-product of cellular metabolism that in higher concentrations can cause oxidative stress. Reactive oxygen species impair the physiological functions of retinal pigment epithelial (RPE) cells, which are known as one major cause of ocular pathologies. Most studies investigating the influence of H2O2 on cells in culture but H2O2 concentrations are not sustained in culture medium. Continuous generation using glucose oxidase (GOx) system allows application of relevant low H2O2 concentrations over physiologically relevant times periods (up to 24 h). Recent findings suggest that bolus and GOx treatments can lead to different cellular response, thus warranting a quantitative comparison between the two approaches. When added as a pulse H2O2 is rapidly depleted. Continuous generation of H2O2 produces different behavior in function of GOx activities. Cytotoxicity analyses show that cells can tolerate short exposure to high H2O2 doses delivered as a pulse but are susceptible to lower continuous doses. Application of hydrogen peroxide causes a concentration-dependent decrease in the intracellular glutathione (GSH) content that was accompanied by a matching decrease in the glutathione peroxide activity and reducing power (FRAP).
VL  - 3
IS  - 3
ER  -

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