Effects of Prolonged Administration of Aqueous Extract of <i>Ocimum gratissimum</i> (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats

Ufele Angela Nwogor

doi:doi:10.11648/j.ajbls.20160403.12

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Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats

Ufele Angela Nwogor

Published in American Journal of Biomedical and Life Sciences (Volume 4, Issue 3)

Received: 31 March 2016 Accepted: 15 April 2016 Published: 28 April 2016

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Abstract

This study was aimed at comparing the antidiabetic and antihyperlipidemic activities of various doses (1.0, 2.0ml and 3.0ml) of aqueous extract of Ocimum gratissimum and glibenclamide (a reference hypoglycemic drug). Ninety (90) male wistar albino rats were used. The rats were divided into six groups (A, B, C, D, E and F) of five rats each. The experiment was replicated three times. The first group, A was non diabetic while B to F was made diabetic using alloxan. Results obtained showed that there was a significant difference (p<0.05) in blood glucose level (mg/dl) following the administration of Ocimum gratissimum leaf extract in groups D and E (139.96±7.2mg/dl and 106.64±4.30mg/dl) when compared to the diabetic control group B (299.34±1.30mg/dl). Administration of glibenclamide (antidiabetic drug) also resulted in a significant drop in the blood glucose levels between week 1 and 4. The effects of Ocimum gratissimum and glibenclamide on the blood glucose level are significantly the same at the final week (week 4). The effect of 2.0ml and 3.0ml (groups C and D) of aqueous extract of Ocimum gratissimum on total cholesterol, triglycerides and low density lipoprotein cholesterol (LDL-C) compared favourably with the reference drug (glibenclamide) whereby they all have significant difference (P<0.05) on these biochemical parameters when compared to the diabetic control. All doses of Ocimum gratissimum aqueous leaf extract increased the level of high density lipoprotein cholesterol (37.41±1.07mg/dl, 42.09±1.22mg/dl and 49.76±0.63mg/dl) when compared to the diabetic control group (32.27±1.68). The effect of highest dose (3.0ml) of Ocimum gratissimum extract on high density lipoprotein cholesterol (HDL-C) (49.76±0.63) compares favourably with the effect seen on the reference drug (glibenclamide) (50.15±1.17). The results suggest that the leaf aqueous extract of Ocimum gratissimum at 3.0ml dosage has antidiabetic and antihyperlipidemic effect and would be of benefit in the treatment and management of diabetes mellitus, controlling the blood sugar level as well as in preventing or delaying the onset of diabetes mellitus.

Published in	American Journal of Biomedical and Life Sciences (Volume 4, Issue 3)
DOI	10.11648/j.ajbls.20160403.12
Page(s)	30-34
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Albino Rats, Ocimum gratissimum, Blood Glucose and Lipoprotein Cholesterol

References

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[2]	Celso, V., Nakamar, I. E., Tania, U. J. (2005). Anti bacteria activity of Ocimum gratissimum essential oil. Momarias do mitituto oswaldo cruz, 94(5): 675-678.
[3]	Chang, X., Jorgensen, A. M., Bardium, P., Led, J. J. (1997). Solution structures of the R6 human insulin hexamer. Biochemistry. 36(31): 9409-22.
[4]	David G. G, (2011) Greenspan’s basic and clinical endocrinology (9^th edition) New York pp 45-48.
[5]	Ezekwe, C. I, Obidoa, O (2001). Biochemical effect of Vernonia amygdalina on rats liver microsome. Nigeria Journal of Biochemical Molecular Biology, 16: 1745-1798.
[6]	Gibbons, G. F. (1998). Hyperlipidemia of diabetes. Clinical Science.71: 477-486.
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[9]	Krinke, G. J. (2000) History, strains and models. The Laboratory Rat (Handbook of exp. Animals). Academic Press. Pp 3-16.
[10]	Ladeji, O., Omekarah, I., Solomon, M. S. (2003) Hypoglycemic properties of aqueous bark extract of Oeiba pentandra in streptozotocin–induced diabetic rats. Journal of Ethnopharmacology, 84: 139-142.
[11]	Lenzen, S. (2008). The mechenisms of alloxan and streptozotocin induced diabetes. Diabetologia, 51(2): 216-226.
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[16]	Ortega, F. J., Gimeno-Bayon, J., Espinosa-Parrilla, J. F., Carrasco, J. L., Batlle, M., Fugliese, M., Mahu, N., Rodriguez, M.J. (2012). ATP-dependent potassium channel blockade strengthens microglial neuroprotection after hypoxia-ischemia in rats. Experimental Neurology, 235(1): 282–296.
[17]	Pessoa, L. M., Morais, S. M., Bevilaqua, C. L., Luciano, J. H. (2002). Anthelmintic activity of essential oil of Ocimum gratissimum and eugenol against Haemonchus contortus. Veterinary Parasitology, 109: 59-63.
[18]	Richmond, W. O. (1973). Cholesterol enzymatic colorimetric test, Chop-PAP method of estimation of total cholesterol in serum. Clinical Chemistry, 191: 1350-1356.
[19]	Rotimi, S. O., Omotosho, O. E., Rotimi, O. A. (2011). Persistence of acidosis in alloxan-induced diabetic rats treated with the juice of Asystasia gangetica leaves. Pharmacognosy Magazine, 8(7): 25-30.
[20]	Socorro, V. F., Madaira, U. C., David, N. (2002). Relaxant effect of the essential oil of Ocimum gratissimum on isolated ileum of the guinea pig. Journal of Ethnopharmacology, 81(1): 3-5.
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Ufele Angela Nwogor. (2016). Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats. American Journal of Biomedical and Life Sciences, 4(3), 30-34. https://doi.org/10.11648/j.ajbls.20160403.12

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Ufele Angela Nwogor. Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats. Am. J. Biomed. Life Sci. 2016, 4(3), 30-34. doi: 10.11648/j.ajbls.20160403.12

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AMA Style

Ufele Angela Nwogor. Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats. Am J Biomed Life Sci. 2016;4(3):30-34. doi: 10.11648/j.ajbls.20160403.12

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@article{10.11648/j.ajbls.20160403.12,
  author = {Ufele Angela Nwogor},
  title = {Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {4},
  number = {3},
  pages = {30-34},
  doi = {10.11648/j.ajbls.20160403.12},
  url = {https://doi.org/10.11648/j.ajbls.20160403.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160403.12},
  abstract = {This study was aimed at comparing the antidiabetic and antihyperlipidemic activities of various doses (1.0, 2.0ml and 3.0ml) of aqueous extract of Ocimum gratissimum and glibenclamide (a reference hypoglycemic drug). Ninety (90) male wistar albino rats were used. The rats were divided into six groups (A, B, C, D, E and F) of five rats each. The experiment was replicated three times. The first group, A was non diabetic while B to F was made diabetic using alloxan. Results obtained showed that there was a significant difference (pOcimum gratissimum leaf extract in groups D and E (139.96±7.2mg/dl and 106.64±4.30mg/dl) when compared to the diabetic control group B (299.34±1.30mg/dl). Administration of glibenclamide (antidiabetic drug) also resulted in a significant drop in the blood glucose levels between week 1 and 4. The effects of Ocimum gratissimum and glibenclamide on the blood glucose level are significantly the same at the final week (week 4). The effect of 2.0ml and 3.0ml (groups C and D) of aqueous extract of Ocimum gratissimum on total cholesterol, triglycerides and low density lipoprotein cholesterol (LDL-C) compared favourably with the reference drug (glibenclamide) whereby they all have significant difference (POcimum gratissimum aqueous leaf extract increased the level of high density lipoprotein cholesterol (37.41±1.07mg/dl, 42.09±1.22mg/dl and 49.76±0.63mg/dl) when compared to the diabetic control group (32.27±1.68). The effect of highest dose (3.0ml) of Ocimum gratissimum extract on high density lipoprotein cholesterol (HDL-C) (49.76±0.63) compares favourably with the effect seen on the reference drug (glibenclamide) (50.15±1.17). The results suggest that the leaf aqueous extract of Ocimum gratissimum at 3.0ml dosage has antidiabetic and antihyperlipidemic effect and would be of benefit in the treatment and management of diabetes mellitus, controlling the blood sugar level as well as in preventing or delaying the onset of diabetes mellitus.},
 year = {2016}
}

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TY  - JOUR
T1  - Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats
AU  - Ufele Angela Nwogor
Y1  - 2016/04/28
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbls.20160403.12
DO  - 10.11648/j.ajbls.20160403.12
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 30
EP  - 34
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20160403.12
AB  - This study was aimed at comparing the antidiabetic and antihyperlipidemic activities of various doses (1.0, 2.0ml and 3.0ml) of aqueous extract of Ocimum gratissimum and glibenclamide (a reference hypoglycemic drug). Ninety (90) male wistar albino rats were used. The rats were divided into six groups (A, B, C, D, E and F) of five rats each. The experiment was replicated three times. The first group, A was non diabetic while B to F was made diabetic using alloxan. Results obtained showed that there was a significant difference (pOcimum gratissimum leaf extract in groups D and E (139.96±7.2mg/dl and 106.64±4.30mg/dl) when compared to the diabetic control group B (299.34±1.30mg/dl). Administration of glibenclamide (antidiabetic drug) also resulted in a significant drop in the blood glucose levels between week 1 and 4. The effects of Ocimum gratissimum and glibenclamide on the blood glucose level are significantly the same at the final week (week 4). The effect of 2.0ml and 3.0ml (groups C and D) of aqueous extract of Ocimum gratissimum on total cholesterol, triglycerides and low density lipoprotein cholesterol (LDL-C) compared favourably with the reference drug (glibenclamide) whereby they all have significant difference (POcimum gratissimum aqueous leaf extract increased the level of high density lipoprotein cholesterol (37.41±1.07mg/dl, 42.09±1.22mg/dl and 49.76±0.63mg/dl) when compared to the diabetic control group (32.27±1.68). The effect of highest dose (3.0ml) of Ocimum gratissimum extract on high density lipoprotein cholesterol (HDL-C) (49.76±0.63) compares favourably with the effect seen on the reference drug (glibenclamide) (50.15±1.17). The results suggest that the leaf aqueous extract of Ocimum gratissimum at 3.0ml dosage has antidiabetic and antihyperlipidemic effect and would be of benefit in the treatment and management of diabetes mellitus, controlling the blood sugar level as well as in preventing or delaying the onset of diabetes mellitus.
VL  - 4
IS  - 3
ER  -

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Author Information

Ufele Angela Nwogor

Zoology Department, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

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Ufele Angela Nwogor. (2016). Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats. American Journal of Biomedical and Life Sciences, 4(3), 30-34. https://doi.org/10.11648/j.ajbls.20160403.12

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ACS Style

Ufele Angela Nwogor. Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats. Am. J. Biomed. Life Sci. 2016, 4(3), 30-34. doi: 10.11648/j.ajbls.20160403.12

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AMA Style

Ufele Angela Nwogor. Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats. Am J Biomed Life Sci. 2016;4(3):30-34. doi: 10.11648/j.ajbls.20160403.12

Copy | Download

@article{10.11648/j.ajbls.20160403.12,
  author = {Ufele Angela Nwogor},
  title = {Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {4},
  number = {3},
  pages = {30-34},
  doi = {10.11648/j.ajbls.20160403.12},
  url = {https://doi.org/10.11648/j.ajbls.20160403.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160403.12},
  abstract = {This study was aimed at comparing the antidiabetic and antihyperlipidemic activities of various doses (1.0, 2.0ml and 3.0ml) of aqueous extract of Ocimum gratissimum and glibenclamide (a reference hypoglycemic drug). Ninety (90) male wistar albino rats were used. The rats were divided into six groups (A, B, C, D, E and F) of five rats each. The experiment was replicated three times. The first group, A was non diabetic while B to F was made diabetic using alloxan. Results obtained showed that there was a significant difference (pOcimum gratissimum leaf extract in groups D and E (139.96±7.2mg/dl and 106.64±4.30mg/dl) when compared to the diabetic control group B (299.34±1.30mg/dl). Administration of glibenclamide (antidiabetic drug) also resulted in a significant drop in the blood glucose levels between week 1 and 4. The effects of Ocimum gratissimum and glibenclamide on the blood glucose level are significantly the same at the final week (week 4). The effect of 2.0ml and 3.0ml (groups C and D) of aqueous extract of Ocimum gratissimum on total cholesterol, triglycerides and low density lipoprotein cholesterol (LDL-C) compared favourably with the reference drug (glibenclamide) whereby they all have significant difference (POcimum gratissimum aqueous leaf extract increased the level of high density lipoprotein cholesterol (37.41±1.07mg/dl, 42.09±1.22mg/dl and 49.76±0.63mg/dl) when compared to the diabetic control group (32.27±1.68). The effect of highest dose (3.0ml) of Ocimum gratissimum extract on high density lipoprotein cholesterol (HDL-C) (49.76±0.63) compares favourably with the effect seen on the reference drug (glibenclamide) (50.15±1.17). The results suggest that the leaf aqueous extract of Ocimum gratissimum at 3.0ml dosage has antidiabetic and antihyperlipidemic effect and would be of benefit in the treatment and management of diabetes mellitus, controlling the blood sugar level as well as in preventing or delaying the onset of diabetes mellitus.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Effects of Prolonged Administration of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats
AU  - Ufele Angela Nwogor
Y1  - 2016/04/28
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbls.20160403.12
DO  - 10.11648/j.ajbls.20160403.12
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 30
EP  - 34
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20160403.12
AB  - This study was aimed at comparing the antidiabetic and antihyperlipidemic activities of various doses (1.0, 2.0ml and 3.0ml) of aqueous extract of Ocimum gratissimum and glibenclamide (a reference hypoglycemic drug). Ninety (90) male wistar albino rats were used. The rats were divided into six groups (A, B, C, D, E and F) of five rats each. The experiment was replicated three times. The first group, A was non diabetic while B to F was made diabetic using alloxan. Results obtained showed that there was a significant difference (pOcimum gratissimum leaf extract in groups D and E (139.96±7.2mg/dl and 106.64±4.30mg/dl) when compared to the diabetic control group B (299.34±1.30mg/dl). Administration of glibenclamide (antidiabetic drug) also resulted in a significant drop in the blood glucose levels between week 1 and 4. The effects of Ocimum gratissimum and glibenclamide on the blood glucose level are significantly the same at the final week (week 4). The effect of 2.0ml and 3.0ml (groups C and D) of aqueous extract of Ocimum gratissimum on total cholesterol, triglycerides and low density lipoprotein cholesterol (LDL-C) compared favourably with the reference drug (glibenclamide) whereby they all have significant difference (POcimum gratissimum aqueous leaf extract increased the level of high density lipoprotein cholesterol (37.41±1.07mg/dl, 42.09±1.22mg/dl and 49.76±0.63mg/dl) when compared to the diabetic control group (32.27±1.68). The effect of highest dose (3.0ml) of Ocimum gratissimum extract on high density lipoprotein cholesterol (HDL-C) (49.76±0.63) compares favourably with the effect seen on the reference drug (glibenclamide) (50.15±1.17). The results suggest that the leaf aqueous extract of Ocimum gratissimum at 3.0ml dosage has antidiabetic and antihyperlipidemic effect and would be of benefit in the treatment and management of diabetes mellitus, controlling the blood sugar level as well as in preventing or delaying the onset of diabetes mellitus.
VL  - 4
IS  - 3
ER  -

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