Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes

Mohamed Fouad; Maher Boraie

doi:doi:10.11648/j.ajim.20150301.11

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Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes

Mohamed Fouad, Maher Boraie

Published in American Journal of Internal Medicine (Volume 3, Issue 1)

Received: 18 January 2015 Accepted: 29 January 2015 Published: 6 February 2015

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Abstract

Background: Early detection of diabetic nephropathy (DN) is important. Matrix metalloproteinases-2 (MMP-2) regulates a variety of cellular functions including apoptosis and angiogenesis. Diabetic environment stimulates the secretion of MMP-2 that is considered to participate in DN. Objectives: We conducted this study to investigate the level of MMP-2 as a potential marker of early nephropathy in type 1 diabetes. Methods: The total number of the study was 300 participants, among them 100 participants, were healthy volunteers control group with comparable age and sex to other participants (Group 1). The remaining 200 participants were suffering from type 1 diabetes and were categorized according to duration of diabetes into 100 patients had disease duration less than 5 years and all of them non microalbuimnuric (Group 2) and the last 100 patients had disease duration more than 5 years (Group 3). All subjects were submitted to complete clinical examination; routine laboratory investigations, including; random blood sugar (RBS); glycosylated hemoglobin (HbA1C) and quantitative determination of microalbuminuria (MA) for DN. Specific laboratory investigation for MMP-2 by enzyme-linked immunosorbent assay. Results: RBS and HbA-1c were significantly higher in group 3 than group 2. MA significantly detected only in group 3. MMP-2 was significantly higher in group 3 than the other groups 1, 2 and in the meantime significantly higher in group 2 than 1. MMP-2 starts to rise early before the onset of MA in group 2. Eventually duration of diabetes, RBS, HbA1c and MA were positively correlated with the MMP-2 level. (r=0. 44; P<0.05), (r=0. 43; P<0.05), (r=0. 58; P<0.05) and (r=0. 71; P<0.001) respectively. MMP-2 cutoff level of ≥ 311 ng/ml had a greater sensitivity and specificity for identifying MA (P<0.001). Conclusion: MMP-2 level pre-date the clinical evidence of MA, may serve as an important predictor for early development of DN and a potential marker of severity.

Published in	American Journal of Internal Medicine (Volume 3, Issue 1)
DOI	10.11648/j.ajim.20150301.11
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Matrix Metalloprteinase-2, Type1 Diabetes Mellitus, Microalbuminuria, Diabetic Nephropathy

References

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APA Style

Mohamed Fouad, Maher Boraie. (2015). Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes. American Journal of Internal Medicine, 3(1), 1-5. https://doi.org/10.11648/j.ajim.20150301.11

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ACS Style

Mohamed Fouad; Maher Boraie. Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes. Am. J. Intern. Med. 2015, 3(1), 1-5. doi: 10.11648/j.ajim.20150301.11

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AMA Style

Mohamed Fouad, Maher Boraie. Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes. Am J Intern Med. 2015;3(1):1-5. doi: 10.11648/j.ajim.20150301.11

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@article{10.11648/j.ajim.20150301.11,
  author = {Mohamed Fouad and Maher Boraie},
  title = {Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes},
  journal = {American Journal of Internal Medicine},
  volume = {3},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ajim.20150301.11},
  url = {https://doi.org/10.11648/j.ajim.20150301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20150301.11},
  abstract = {Background: Early detection of diabetic nephropathy (DN) is important. Matrix metalloproteinases-2 (MMP-2) regulates a variety of cellular functions including apoptosis and angiogenesis. Diabetic environment stimulates the secretion of MMP-2 that is considered to participate in DN. Objectives: We conducted this study to investigate the level of MMP-2 as a potential marker of early nephropathy in type 1 diabetes. Methods: The total number of the study was 300 participants, among them 100 participants, were healthy volunteers control group with comparable age and sex to other participants (Group 1). The remaining 200 participants were suffering from type 1 diabetes and were categorized according to duration of diabetes into 100 patients had disease duration less than 5 years and all of them non microalbuimnuric (Group 2) and the last 100 patients had disease duration more than 5 years (Group 3). All subjects were submitted to complete clinical examination; routine laboratory investigations, including; random blood sugar (RBS); glycosylated hemoglobin (HbA1C) and quantitative determination of microalbuminuria (MA) for DN. Specific laboratory investigation for MMP-2 by enzyme-linked immunosorbent assay. Results: RBS and HbA-1c were significantly higher in group 3 than group 2. MA significantly detected only in group 3. MMP-2 was significantly higher in group 3 than the other groups 1, 2 and in the meantime significantly higher in group 2 than 1. MMP-2 starts to rise early before the onset of MA in group 2. Eventually duration of diabetes, RBS, HbA1c and MA were positively correlated with the MMP-2 level. (r=0. 44; P<0.05), (r=0. 43; P<0.05), (r=0. 58; P<0.05) and (r=0. 71; P<0.001) respectively. MMP-2 cutoff level of ≥ 311 ng/ml had a greater sensitivity and specificity for identifying MA (P<0.001). Conclusion: MMP-2 level pre-date the clinical evidence of MA, may serve as an important predictor for early development of DN and a potential marker of severity.},
 year = {2015}
}

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TY  - JOUR
T1  - Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes
AU  - Mohamed Fouad
AU  - Maher Boraie
Y1  - 2015/02/06
PY  - 2015
N1  - https://doi.org/10.11648/j.ajim.20150301.11
DO  - 10.11648/j.ajim.20150301.11
T2  - American Journal of Internal Medicine
JF  - American Journal of Internal Medicine
JO  - American Journal of Internal Medicine
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2330-4324
UR  - https://doi.org/10.11648/j.ajim.20150301.11
AB  - Background: Early detection of diabetic nephropathy (DN) is important. Matrix metalloproteinases-2 (MMP-2) regulates a variety of cellular functions including apoptosis and angiogenesis. Diabetic environment stimulates the secretion of MMP-2 that is considered to participate in DN. Objectives: We conducted this study to investigate the level of MMP-2 as a potential marker of early nephropathy in type 1 diabetes. Methods: The total number of the study was 300 participants, among them 100 participants, were healthy volunteers control group with comparable age and sex to other participants (Group 1). The remaining 200 participants were suffering from type 1 diabetes and were categorized according to duration of diabetes into 100 patients had disease duration less than 5 years and all of them non microalbuimnuric (Group 2) and the last 100 patients had disease duration more than 5 years (Group 3). All subjects were submitted to complete clinical examination; routine laboratory investigations, including; random blood sugar (RBS); glycosylated hemoglobin (HbA1C) and quantitative determination of microalbuminuria (MA) for DN. Specific laboratory investigation for MMP-2 by enzyme-linked immunosorbent assay. Results: RBS and HbA-1c were significantly higher in group 3 than group 2. MA significantly detected only in group 3. MMP-2 was significantly higher in group 3 than the other groups 1, 2 and in the meantime significantly higher in group 2 than 1. MMP-2 starts to rise early before the onset of MA in group 2. Eventually duration of diabetes, RBS, HbA1c and MA were positively correlated with the MMP-2 level. (r=0. 44; P<0.05), (r=0. 43; P<0.05), (r=0. 58; P<0.05) and (r=0. 71; P<0.001) respectively. MMP-2 cutoff level of ≥ 311 ng/ml had a greater sensitivity and specificity for identifying MA (P<0.001). Conclusion: MMP-2 level pre-date the clinical evidence of MA, may serve as an important predictor for early development of DN and a potential marker of severity.
VL  - 3
IS  - 1
ER  -

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Author Information

Mohamed Fouad

Nephrology unit, Internal Medicine Departments, Zagazig University Hospital, Zagazig, Egypt
Maher Boraie

Clinical Pathology Departments, Zagazig University Hospital, Zagazig, Egypt

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APA Style

Mohamed Fouad, Maher Boraie. (2015). Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes. American Journal of Internal Medicine, 3(1), 1-5. https://doi.org/10.11648/j.ajim.20150301.11

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ACS Style

Mohamed Fouad; Maher Boraie. Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes. Am. J. Intern. Med. 2015, 3(1), 1-5. doi: 10.11648/j.ajim.20150301.11

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AMA Style

Mohamed Fouad, Maher Boraie. Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes. Am J Intern Med. 2015;3(1):1-5. doi: 10.11648/j.ajim.20150301.11

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@article{10.11648/j.ajim.20150301.11,
  author = {Mohamed Fouad and Maher Boraie},
  title = {Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes},
  journal = {American Journal of Internal Medicine},
  volume = {3},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ajim.20150301.11},
  url = {https://doi.org/10.11648/j.ajim.20150301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20150301.11},
  abstract = {Background: Early detection of diabetic nephropathy (DN) is important. Matrix metalloproteinases-2 (MMP-2) regulates a variety of cellular functions including apoptosis and angiogenesis. Diabetic environment stimulates the secretion of MMP-2 that is considered to participate in DN. Objectives: We conducted this study to investigate the level of MMP-2 as a potential marker of early nephropathy in type 1 diabetes. Methods: The total number of the study was 300 participants, among them 100 participants, were healthy volunteers control group with comparable age and sex to other participants (Group 1). The remaining 200 participants were suffering from type 1 diabetes and were categorized according to duration of diabetes into 100 patients had disease duration less than 5 years and all of them non microalbuimnuric (Group 2) and the last 100 patients had disease duration more than 5 years (Group 3). All subjects were submitted to complete clinical examination; routine laboratory investigations, including; random blood sugar (RBS); glycosylated hemoglobin (HbA1C) and quantitative determination of microalbuminuria (MA) for DN. Specific laboratory investigation for MMP-2 by enzyme-linked immunosorbent assay. Results: RBS and HbA-1c were significantly higher in group 3 than group 2. MA significantly detected only in group 3. MMP-2 was significantly higher in group 3 than the other groups 1, 2 and in the meantime significantly higher in group 2 than 1. MMP-2 starts to rise early before the onset of MA in group 2. Eventually duration of diabetes, RBS, HbA1c and MA were positively correlated with the MMP-2 level. (r=0. 44; P<0.05), (r=0. 43; P<0.05), (r=0. 58; P<0.05) and (r=0. 71; P<0.001) respectively. MMP-2 cutoff level of ≥ 311 ng/ml had a greater sensitivity and specificity for identifying MA (P<0.001). Conclusion: MMP-2 level pre-date the clinical evidence of MA, may serve as an important predictor for early development of DN and a potential marker of severity.},
 year = {2015}
}

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TY  - JOUR
T1  - Matrix Metalloproteinase-2 as Potential Marker of Early Nephropathy in Type 1 Diabetes
AU  - Mohamed Fouad
AU  - Maher Boraie
Y1  - 2015/02/06
PY  - 2015
N1  - https://doi.org/10.11648/j.ajim.20150301.11
DO  - 10.11648/j.ajim.20150301.11
T2  - American Journal of Internal Medicine
JF  - American Journal of Internal Medicine
JO  - American Journal of Internal Medicine
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2330-4324
UR  - https://doi.org/10.11648/j.ajim.20150301.11
AB  - Background: Early detection of diabetic nephropathy (DN) is important. Matrix metalloproteinases-2 (MMP-2) regulates a variety of cellular functions including apoptosis and angiogenesis. Diabetic environment stimulates the secretion of MMP-2 that is considered to participate in DN. Objectives: We conducted this study to investigate the level of MMP-2 as a potential marker of early nephropathy in type 1 diabetes. Methods: The total number of the study was 300 participants, among them 100 participants, were healthy volunteers control group with comparable age and sex to other participants (Group 1). The remaining 200 participants were suffering from type 1 diabetes and were categorized according to duration of diabetes into 100 patients had disease duration less than 5 years and all of them non microalbuimnuric (Group 2) and the last 100 patients had disease duration more than 5 years (Group 3). All subjects were submitted to complete clinical examination; routine laboratory investigations, including; random blood sugar (RBS); glycosylated hemoglobin (HbA1C) and quantitative determination of microalbuminuria (MA) for DN. Specific laboratory investigation for MMP-2 by enzyme-linked immunosorbent assay. Results: RBS and HbA-1c were significantly higher in group 3 than group 2. MA significantly detected only in group 3. MMP-2 was significantly higher in group 3 than the other groups 1, 2 and in the meantime significantly higher in group 2 than 1. MMP-2 starts to rise early before the onset of MA in group 2. Eventually duration of diabetes, RBS, HbA1c and MA were positively correlated with the MMP-2 level. (r=0. 44; P<0.05), (r=0. 43; P<0.05), (r=0. 58; P<0.05) and (r=0. 71; P<0.001) respectively. MMP-2 cutoff level of ≥ 311 ng/ml had a greater sensitivity and specificity for identifying MA (P<0.001). Conclusion: MMP-2 level pre-date the clinical evidence of MA, may serve as an important predictor for early development of DN and a potential marker of severity.
VL  - 3
IS  - 1
ER  -

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