In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract

Yohannes Weldemariam Getahun; Afework Mulugeta Bezabh

doi:doi:10.11648/j.ajls.20150303.12

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In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract

Yohannes Weldemariam Getahun, Afework Mulugeta Bezabh

Published in American Journal of Life Sciences (Volume 3, Issue 3)

Received: 3 March 2015 Accepted: 18 March 2015 Published: 27 April 2015

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Abstract

Unlike synthetic drugs plants have different phytochemical constituents which can act collectively by which helminthes cannot resist them or there could be active constituent(s) in the plant with superior potency. The aim of this study was to investigate the anthelmintic activity of both crude and fractions of Plumbago zeylanica root extract for the purpose of finding long lasting and potent medicinal plant due to significant implications of helminthes in developing countries like Ethiopia. And where traditional medicine is wide spread and of immediate alternative. In the assay, chloroform crude extracts recorded less paralysis and death time than ethanolic crude extracts. Then crude extract was subjected to column chromatography from which nine pure compounds were isolated. In addition, the isolated compounds were higher in their anthelmintic activity than crude extracts at almost all concentrations. Both crude and fractions paralyse and kill the worms with less time than that of the positive control and even less than 10 fold especially at low concentrations in case of chloroform extracts. The findings here on anthelmintic activity of the root at lower concentrations are significant and for the first time. If in vivo data are included the plant can be used as long lasting drug for helminthes.

Published in	American Journal of Life Sciences (Volume 3, Issue 3)
DOI	10.11648/j.ajls.20150303.12
Page(s)	134-142
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Plumbago Zeylanica L, In Vitro Test, Anthelmintic Activity, Extraction, Fractionation

References

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Yohannes Weldemariam Getahun, Afework Mulugeta Bezabh. (2015). In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract. American Journal of Life Sciences, 3(3), 134-142. https://doi.org/10.11648/j.ajls.20150303.12

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ACS Style

Yohannes Weldemariam Getahun; Afework Mulugeta Bezabh. In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract. Am. J. Life Sci. 2015, 3(3), 134-142. doi: 10.11648/j.ajls.20150303.12

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AMA Style

Yohannes Weldemariam Getahun, Afework Mulugeta Bezabh. In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract. Am J Life Sci. 2015;3(3):134-142. doi: 10.11648/j.ajls.20150303.12

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@article{10.11648/j.ajls.20150303.12,
  author = {Yohannes Weldemariam Getahun and Afework Mulugeta Bezabh},
  title = {In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {3},
  pages = {134-142},
  doi = {10.11648/j.ajls.20150303.12},
  url = {https://doi.org/10.11648/j.ajls.20150303.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150303.12},
  abstract = {Unlike synthetic drugs plants have different phytochemical constituents which can act collectively by which helminthes cannot resist them or there could be active constituent(s) in the plant with superior potency. The aim of this study was to investigate the anthelmintic activity of both crude and fractions of Plumbago zeylanica root extract for the purpose of finding long lasting and potent medicinal plant due to significant implications of helminthes in developing countries like Ethiopia. And where traditional medicine is wide spread and of immediate alternative. In the assay, chloroform crude extracts recorded less paralysis and death time than ethanolic crude extracts. Then crude extract was subjected to column chromatography from which nine pure compounds were isolated. In addition, the isolated compounds were higher in their anthelmintic activity than crude extracts at almost all concentrations. Both crude and fractions paralyse and kill the worms with less time than that of the positive control and even less than 10 fold especially at low concentrations in case of chloroform extracts. The findings here on anthelmintic activity of the root at lower concentrations are significant and for the first time.  If in vivo data are included the plant can be used as long lasting drug for helminthes.},
 year = {2015}
}

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TY  - JOUR
T1  - In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract
AU  - Yohannes Weldemariam Getahun
AU  - Afework Mulugeta Bezabh
Y1  - 2015/04/27
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.20150303.12
DO  - 10.11648/j.ajls.20150303.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 134
EP  - 142
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20150303.12
AB  - Unlike synthetic drugs plants have different phytochemical constituents which can act collectively by which helminthes cannot resist them or there could be active constituent(s) in the plant with superior potency. The aim of this study was to investigate the anthelmintic activity of both crude and fractions of Plumbago zeylanica root extract for the purpose of finding long lasting and potent medicinal plant due to significant implications of helminthes in developing countries like Ethiopia. And where traditional medicine is wide spread and of immediate alternative. In the assay, chloroform crude extracts recorded less paralysis and death time than ethanolic crude extracts. Then crude extract was subjected to column chromatography from which nine pure compounds were isolated. In addition, the isolated compounds were higher in their anthelmintic activity than crude extracts at almost all concentrations. Both crude and fractions paralyse and kill the worms with less time than that of the positive control and even less than 10 fold especially at low concentrations in case of chloroform extracts. The findings here on anthelmintic activity of the root at lower concentrations are significant and for the first time.  If in vivo data are included the plant can be used as long lasting drug for helminthes.
VL  - 3
IS  - 3
ER  -

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Author Information

Yohannes Weldemariam Getahun

Department of Chemistry, Adigrat University, Adigrat, Tigray, Ethiopia
Afework Mulugeta Bezabh

Department of Public Health, Mekelle University, Mekelle, Tigray, Ethiopia

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APA Style

Yohannes Weldemariam Getahun, Afework Mulugeta Bezabh. (2015). In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract. American Journal of Life Sciences, 3(3), 134-142. https://doi.org/10.11648/j.ajls.20150303.12

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ACS Style

Yohannes Weldemariam Getahun; Afework Mulugeta Bezabh. In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract. Am. J. Life Sci. 2015, 3(3), 134-142. doi: 10.11648/j.ajls.20150303.12

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AMA Style

Yohannes Weldemariam Getahun, Afework Mulugeta Bezabh. In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract. Am J Life Sci. 2015;3(3):134-142. doi: 10.11648/j.ajls.20150303.12

Copy | Download

@article{10.11648/j.ajls.20150303.12,
  author = {Yohannes Weldemariam Getahun and Afework Mulugeta Bezabh},
  title = {In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract},
  journal = {American Journal of Life Sciences},
  volume = {3},
  number = {3},
  pages = {134-142},
  doi = {10.11648/j.ajls.20150303.12},
  url = {https://doi.org/10.11648/j.ajls.20150303.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150303.12},
  abstract = {Unlike synthetic drugs plants have different phytochemical constituents which can act collectively by which helminthes cannot resist them or there could be active constituent(s) in the plant with superior potency. The aim of this study was to investigate the anthelmintic activity of both crude and fractions of Plumbago zeylanica root extract for the purpose of finding long lasting and potent medicinal plant due to significant implications of helminthes in developing countries like Ethiopia. And where traditional medicine is wide spread and of immediate alternative. In the assay, chloroform crude extracts recorded less paralysis and death time than ethanolic crude extracts. Then crude extract was subjected to column chromatography from which nine pure compounds were isolated. In addition, the isolated compounds were higher in their anthelmintic activity than crude extracts at almost all concentrations. Both crude and fractions paralyse and kill the worms with less time than that of the positive control and even less than 10 fold especially at low concentrations in case of chloroform extracts. The findings here on anthelmintic activity of the root at lower concentrations are significant and for the first time.  If in vivo data are included the plant can be used as long lasting drug for helminthes.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - In Vitro Anthelmintic Efficacy of Fractions from Plumbago zeylanica L (Family- Plumbaginnaceae) Root Extract
AU  - Yohannes Weldemariam Getahun
AU  - Afework Mulugeta Bezabh
Y1  - 2015/04/27
PY  - 2015
N1  - https://doi.org/10.11648/j.ajls.20150303.12
DO  - 10.11648/j.ajls.20150303.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 134
EP  - 142
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20150303.12
AB  - Unlike synthetic drugs plants have different phytochemical constituents which can act collectively by which helminthes cannot resist them or there could be active constituent(s) in the plant with superior potency. The aim of this study was to investigate the anthelmintic activity of both crude and fractions of Plumbago zeylanica root extract for the purpose of finding long lasting and potent medicinal plant due to significant implications of helminthes in developing countries like Ethiopia. And where traditional medicine is wide spread and of immediate alternative. In the assay, chloroform crude extracts recorded less paralysis and death time than ethanolic crude extracts. Then crude extract was subjected to column chromatography from which nine pure compounds were isolated. In addition, the isolated compounds were higher in their anthelmintic activity than crude extracts at almost all concentrations. Both crude and fractions paralyse and kill the worms with less time than that of the positive control and even less than 10 fold especially at low concentrations in case of chloroform extracts. The findings here on anthelmintic activity of the root at lower concentrations are significant and for the first time.  If in vivo data are included the plant can be used as long lasting drug for helminthes.
VL  - 3
IS  - 3
ER  -

Copy | Download