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In Vitro Antimicrobial Efficacy of Fractions from Onion (Allium Cepa) Leaves Extract from Wukro, Ethiopia

Received: 30 July 2015     Accepted: 6 August 2015     Published: 2 September 2015
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Abstract

To examine the in vitro anti bacterial activities of the ethanol extract of fresh leaves of Alliumcepa (onion) and to determine and quantify the phenol compounds of the investigated plantparts. This study was drifting out at the Mekelle university department chemistry and Adigrat pharmaceutical industry, fromMarch2015toApril 2015. Clinical strains of Streptococcus pneumoniae and ethanol extracts of the plant species was used for the antimicrobial study. Thirty grams of the sample was ground, filtrated, and each filtrate mixed with 100 ml ethanol and placed in a shaker for 48 hours. Theethanol was evaporated from the sample, weighed, and subjected to an antibacterial activity test using the agar diffusion method. The high- performance liquid chromatography was used to identify and quantify the phenols extracts of investigated samples. Ethanol extract of the investigated plant parts showed antibacterial activities against different pathogenicbacteria. Leaf extracts of Alliumcepashowed the highest antibacterial activity and contains more phenols. Theethanol extract of the tested plants could be considered as analternative source of new antibacterialdrugs.

Published in American Journal of Life Sciences (Volume 3, Issue 5)
DOI 10.11648/j.ajls.20150305.15
Page(s) 365-368
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

HPLC, Antimicrobial Empire, Streptococcus Pneumoniae, Medicinal Plants

References
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[17] Sequeira BJ, Vital MJ, Pohlit AM, Pararols IC, Caúper GS. Antibacterialand antifungal activity of extracts and exudates of the Amazonian medicinal treeHimatanthusarticulatus(Vahl)Woodson(commonname:sucuba).MemInstOswaldoCruz.2009; 104: 659-661.
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  • APA Style

    Teklit Gebregiorgis Amabye. (2015). In Vitro Antimicrobial Efficacy of Fractions from Onion (Allium Cepa) Leaves Extract from Wukro, Ethiopia. American Journal of Life Sciences, 3(5), 365-368. https://doi.org/10.11648/j.ajls.20150305.15

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    ACS Style

    Teklit Gebregiorgis Amabye. In Vitro Antimicrobial Efficacy of Fractions from Onion (Allium Cepa) Leaves Extract from Wukro, Ethiopia. Am. J. Life Sci. 2015, 3(5), 365-368. doi: 10.11648/j.ajls.20150305.15

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    AMA Style

    Teklit Gebregiorgis Amabye. In Vitro Antimicrobial Efficacy of Fractions from Onion (Allium Cepa) Leaves Extract from Wukro, Ethiopia. Am J Life Sci. 2015;3(5):365-368. doi: 10.11648/j.ajls.20150305.15

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  • @article{10.11648/j.ajls.20150305.15,
      author = {Teklit Gebregiorgis Amabye},
      title = {In Vitro Antimicrobial Efficacy of Fractions from Onion (Allium Cepa) Leaves Extract from Wukro, Ethiopia},
      journal = {American Journal of Life Sciences},
      volume = {3},
      number = {5},
      pages = {365-368},
      doi = {10.11648/j.ajls.20150305.15},
      url = {https://doi.org/10.11648/j.ajls.20150305.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150305.15},
      abstract = {To examine the in vitro anti bacterial activities of the ethanol extract of fresh leaves of Alliumcepa (onion) and to determine and quantify the phenol compounds of the investigated plantparts. This study was drifting out at the Mekelle university department chemistry and Adigrat pharmaceutical industry, fromMarch2015toApril 2015. Clinical strains of Streptococcus pneumoniae and ethanol extracts of the plant species was used for the antimicrobial study. Thirty grams of the sample was ground, filtrated, and each filtrate mixed with 100 ml ethanol and placed in a shaker for 48 hours. Theethanol was evaporated from the sample, weighed, and subjected to an antibacterial activity test using the agar diffusion method. The high- performance liquid chromatography was used to identify and quantify the phenols extracts of investigated samples. Ethanol extract of the investigated plant parts showed antibacterial activities against different pathogenicbacteria. Leaf extracts of Alliumcepashowed the highest antibacterial activity and contains more phenols. Theethanol extract of the tested plants could be considered as analternative source of new antibacterialdrugs.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - In Vitro Antimicrobial Efficacy of Fractions from Onion (Allium Cepa) Leaves Extract from Wukro, Ethiopia
    AU  - Teklit Gebregiorgis Amabye
    Y1  - 2015/09/02
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajls.20150305.15
    DO  - 10.11648/j.ajls.20150305.15
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 365
    EP  - 368
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20150305.15
    AB  - To examine the in vitro anti bacterial activities of the ethanol extract of fresh leaves of Alliumcepa (onion) and to determine and quantify the phenol compounds of the investigated plantparts. This study was drifting out at the Mekelle university department chemistry and Adigrat pharmaceutical industry, fromMarch2015toApril 2015. Clinical strains of Streptococcus pneumoniae and ethanol extracts of the plant species was used for the antimicrobial study. Thirty grams of the sample was ground, filtrated, and each filtrate mixed with 100 ml ethanol and placed in a shaker for 48 hours. Theethanol was evaporated from the sample, weighed, and subjected to an antibacterial activity test using the agar diffusion method. The high- performance liquid chromatography was used to identify and quantify the phenols extracts of investigated samples. Ethanol extract of the investigated plant parts showed antibacterial activities against different pathogenicbacteria. Leaf extracts of Alliumcepashowed the highest antibacterial activity and contains more phenols. Theethanol extract of the tested plants could be considered as analternative source of new antibacterialdrugs.
    VL  - 3
    IS  - 5
    ER  - 

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Author Information
  • Department of Chemistry, College of Natural and Computational Science, Mekelle University, Mekelle, Ethiopia

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