In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata

Hafizur Rahman; Vijaya B; Soumya Ghosh; Geetika Pant; Sibi G

doi:doi:10.11648/j.ajls.s.2014020601.12

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In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata

Hafizur Rahman, Vijaya B, Soumya Ghosh, Geetika Pant, Sibi G

Published in American Journal of Life Sciences (Volume 2, Issue 6-1)

Received: 13 August 2014 Accepted: 15 August 2014 Published: 24 August 2014

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Abstract

New drug preparations of natural origin are in need due to the numerous side effects and resistance development through the continuous and uncontrolled use of synthetic drugs. Lichens are reported to have manifold biological activities and in this study, antioxidant, hypolipidemic and cytotoxic potential of Parmelia perlata were assessed. Methanolic extract of P. perlata was prepared and used in DPPH assay, total phenolic content and total antioxidant potential assays. In vitro anti-cholesterol and cytotoxic assay using HCT 116 cell lines were performed. The results revealed that P. perlata contain high phenolic contents and antioxidant potential. Simvastatin was used as standard drug and the extracts demonstrated 48% anti-cholesterol activity. MTT assay using colon cancer cell lines (HCT 116) produced dose dependent cytotoxic effect with an IC50 value of 202.1 µg ml-1.

Published in	American Journal of Life Sciences (Volume 2, Issue 6-1) This article belongs to the Special Issue Recent Developments in Health Care through Plants and Microbes
DOI	10.11648/j.ajls.s.2014020601.12
Page(s)	7-10
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Parmelia Perlata, Antioxidant, Anticancer, Anti-Cholesterol, Colon Cancer

References

[1]	F. Danhier, O. Feron, V. Preat, “To exploit the tumor microenvironment: Passive and active tumor targeting of nanocarriers for anti-cancer drug delivery,” J. Control. Release., vol. 148, 35-146, 2010.
[2]	W.M. Zhang, B. Li, L. Han, H.D. Zhang, HD, “Antioxidant activities of extracts from areca (Areca catectu L.) flower, husk and seed,” Afr J Biotechnol., vol. 8: 3887-3892, 2009.
[3]	K. Molnar, E. Farkas, “Current results on biological activities of lichen secondary metabolites: a review,” Journal of Biosciences., vol. 65, 157-173, 2010.
[4]	T. Mitrovic, S. Stamenkovic, V. Cvetkovic, M. Nikolic, S. Tosic, D. Stojicic, “Lichens as source of versatile bioactive compounds,” Biologica Nyssana., vol. 2, 1-6, 2011.
[5]	B.C. Behera, N. Verma, A. Sonone, U. Makhija, “Determination of antioxidative potential of lichen Usnea ghattensis in vitro,” LWT-Food Sci Technol., vol. 39, 80-85, 2006.
[6]	M. Gulluce, A. Aslan, M. Sokmen, F. Sahin, A. Adiquzel, G. Agar, A. Sokmen, “Screening the antioxidant and antimicrobial properties of the lichens Parmelia saxatilis, Platismatia glauca, Ramalina pollinaria, Ramalina polymorpha and Umbilicaria nylanderiana,” Phytomedicine., vol. 13, 515-521, 2006.
[7]	H.D. Bhattarai, B. Paudel, S.G. Hong, H.K. Lee, J.H. Yim, “Thin layer chromatography analysis of antioxidant constituents of lichens from Antarctica,” J Nat Med., vol. 62, 481-484, 2008.
[8]	B. Paudel, H.D. Bhattarai, J.S. Lee, S.G. Hong, H.W. Shin, et al. “Antioxidant activity of polar lichens from King George Island (Antarctica),” Polar Biol., vol. 31, 605-608, 2008.
[9]	B.R. Rankovic, M.M. Kosani, T.P. Stanojkovic, “Antioxidant, antimicrobial and anticancer activity of the lichens Cladonia furcata, Lecanora atra and Lecanora muralis,” BMC Complement Altern Med., vol. 11, 97, 2011.
[10]	A. Karagoz, N. Dogruoz, Z. Zeybek, A. Aslan, “Antibacterial activity of some lichen extracts,” J Med Plants Res., vol. 3, 1034-1039, 2009.
[11]	M. Kosanic, B. Rankovic, “Antibacterial and antifungal activity of different lichens extracts and lichen acid,” Res J Biotechnol., vol. 6, 23-26, 2011.
[12]	N.B. Perry, M.H. Benn, N.J. Brennan, E.J. Burgess, G. Ellis, D.J. Galloway, S.D. Lorimer, S. Tangney, “Antimicrobial, antiviral and cytotoxic activity of New Zealand lichens.” Lichenologist., vol. 31, 627-636, 1999.
[13]	C. Bezivin, S. Tomasi, F. Lohezic-Le Devehat, J. Boustie, J. “Cytotoxic activity of some lichen extracts on murine and human cancer cell lines,” Phytomedicine., vol. 10, 499-503, 2003.
[14]	K.R. Kiritikar, B.D. Basu, Indian Medicinal plants. 2nd Ed. M.B. Lalit Mohan Basu, Allahabad, vol. 3, 1956.
[15]	P. Halama, C.V. Haluwin, “Antifungal activity of lichen extracts and lichenic acids,” BioControl, vol. 49, 95-107, 2004.
[16]	T. Tay, T.A. Ozdemir, M. Yilmaz, H. Turk, M. Kivanc, “Evaluation of the antimicrobial activity of the acetone extract of the Lichen Ramalina farinacea and its (+)-usnic acid, norstictic acid, and protocetraric acid constituents,” Journal of Biosciences, vol. 59, 384-388, 2004.
[17]	B. Thippeswamy, N.R. Sushma, K.J. Naveenkumar, “Antimicrobial property of bioactive factor isolated from Parmelia perlata,” International Multidisciplinary Research Journal, vol. 2, 1-5, 2012.
[18]	G. Sibi, V. Apsara, K. Dhananjaya, K.R. Ravikumar and H. Mallesha, “Phytochemical and antibacterial properties of spices against food borne bacteria with special reference to Parmelia perlata,” Global J. Bio-Sci. Biotechnol., vol. 2, 145-149, 2013.
[19]	C.O. Esimone, K.C. Ofokansi, M.U. Adikwu, E.C. Ibezim, D.O. Abonyi, G.N. Odaibo, D.O. Olaleye, “In vitro evaluation of the antiviral activity of extracts from the lichen Parmelia perlata (L.) Ach. against three RNA viruses,” J Infect Dev Ctries, vol. 1, 315-320, 2007.
[20]	S.B. Patil, V.A. Ghadyale, S.S. Taklikar, C.R. Kulkarni, A.U. Arvindekar, “Insulin secretagogue, alpha-glucosidase and antioxidant activity of some selected spices in streptozotocin-induced diabetic rats,” Plant Foods Hum Nutr, vol. 66, 85-90, 2011.
[21]	K. Muller, “Pharmaceutically relevant metabolites from lichens,” Appl. Microbiol Biotechnol., vol. 56, 9-16, 2001.
[22]	M.M. Kosanic, B.R. Rankovic, T.P. Stanojkovic, “Antioxidant, antimicrobial and anticancer activities of three Parmelia species,” J. Sci. Food Agric., vol. 92, 1909-1916, 2012.
[23]	J.A. Correia da Silva, “Action of Parmelia caperata extracts on smooth muscle organs,” Arch. Farmacol. Toxicol., vol. 2, 143-152, 1976.
[24]	M.A. Gyamfi, M. Yonamine, Y. Aniya, “Free radical scavenging action of medicinal herbs from Ghana Thonningia sanguinea on experimentally induced liver injuries,” Gen Pharmacol, vol. 32, 661-667, 1999.
[25]	E.A. Ainsworth, K.M. Gillespie, Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature Protocols, vol. 2, 875- 877, 2007.
[26]	P. Prieto, M. Pineda, M. Aguilar M, “Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: Specific application to the determination of vitamin E,” Analytical Biochemistry, vol. 269, 337-341, 1999.
[27]	D. Iswantini, D. Nurenda dan, P. Sugita, “Fractionation and characterization of active compounds from Bangle (Zingiber Cassumunar Roxb.) as an activator of the enzyme cholesterol oxidase,” Simposium Nasional Kimia Bahan Alam XV. Himpunan Kimia Bahan Alam. Bogor., 2005
[28]	Randox Laboratories, 2009. Cholesterol (Chol) enzymatic endpoint method manual. Available online.
[29]	S.P.M. Crouch, R. Kozlowski, K.J. Slater, J. Fletcher, “The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity”. J. Immunol. Methods vol. 160, 81-88, 1993.
[30]	J.M.C. Gutteridge, “Free radicals in Disease Processes: A Compilation of cause and consequence,” Free Radic. Res. Comm., vol. 19, 141, 1995.
[31]	B. Auddy, F. Ferreira, L. Blasina, F. Lafon, F. Arredondo, F. Dajas, P.C. Tripathi, T. Seal, B. Mukherjee, “Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases,” J Ethnopharmacol., vol. 84, 131-138, 2003.
[32]	B. Sagnia, D. Fedeli, R. Casetti, C. Montesano, G. Falcioni, V. Colizzi, “Antioxidant and Anti-inflammatory activities of extracts from Cassia alata, Eleusine indica, Eremomastax speciosa, Carica papaya and Polyscias fulva medicinal plants collected in Cameroon,” PLoS One, vol. 9, e103999, 2014.
[33]	Cancer Statistics, http://seer.cancer.gov/statfacts/html/colorect.html accessed on Aug, 2014
[34]	N. Manojlovic, B. Rankovic, M. Kosanic, P. Vasiljevic, T. Stanojkovic, “Chemical composition of three Parmelia lichens and antioxidant, antimicrobial and cytotoxic activities of some their major metabolites,” Phytomedicine, vol. 19, 1166-1172, 2012.
[35]	F. Ari, E. Ulukaya, S. Oran, S. Celikler, S. Ozturk, M.Z. Ozel, “Promising anticancer activity of a lichen, Parmelia sulcata Taylor, against breast cancer cell lines and genotoxic effect on human lymphocyte,” Cytotechnology, 2014.
[36]	K.V. Balan, C. Demetzos, J. Prince, K. Dimas, M. Cladaras, Z. Han, J. H. Wyche, P. Pantazis, “Induction of apoptosis in human colon cancer HCT116 cells treated with an extract of the plant product, chios mastic gum,” In Vivo., vol. 19, 93-102, 2005.

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APA Style

Hafizur Rahman, Vijaya B, Soumya Ghosh, Geetika Pant, Sibi G. (2014). In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata. American Journal of Life Sciences, 2(6-1), 7-10. https://doi.org/10.11648/j.ajls.s.2014020601.12

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ACS Style

Hafizur Rahman; Vijaya B; Soumya Ghosh; Geetika Pant; Sibi G. In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata. Am. J. Life Sci. 2014, 2(6-1), 7-10. doi: 10.11648/j.ajls.s.2014020601.12

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AMA Style

Hafizur Rahman, Vijaya B, Soumya Ghosh, Geetika Pant, Sibi G. In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata. Am J Life Sci. 2014;2(6-1):7-10. doi: 10.11648/j.ajls.s.2014020601.12

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@article{10.11648/j.ajls.s.2014020601.12,
  author = {Hafizur Rahman and Vijaya B and Soumya Ghosh and Geetika Pant and Sibi G},
  title = {In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {6-1},
  pages = {7-10},
  doi = {10.11648/j.ajls.s.2014020601.12},
  url = {https://doi.org/10.11648/j.ajls.s.2014020601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2014020601.12},
  abstract = {New drug preparations of natural origin are in need due to the numerous side effects and resistance development through the continuous and uncontrolled use of synthetic drugs. Lichens are reported to have manifold biological activities and  in this study, antioxidant, hypolipidemic and cytotoxic potential of Parmelia perlata were assessed. Methanolic extract of P. perlata was prepared and used in DPPH assay, total phenolic content and total antioxidant potential assays. In vitro anti-cholesterol and cytotoxic assay using HCT 116 cell lines were performed. The results revealed that P. perlata contain high phenolic contents and antioxidant potential. Simvastatin was used as standard drug and the extracts demonstrated 48% anti-cholesterol activity. MTT assay using colon cancer cell lines (HCT 116) produced dose dependent cytotoxic effect with an IC50 value of 202.1 µg ml-1.},
 year = {2014}
}

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TY  - JOUR
T1  - In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata
AU  - Hafizur Rahman
AU  - Vijaya B
AU  - Soumya Ghosh
AU  - Geetika Pant
AU  - Sibi G
Y1  - 2014/08/24
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.s.2014020601.12
DO  - 10.11648/j.ajls.s.2014020601.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 7
EP  - 10
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.s.2014020601.12
AB  - New drug preparations of natural origin are in need due to the numerous side effects and resistance development through the continuous and uncontrolled use of synthetic drugs. Lichens are reported to have manifold biological activities and  in this study, antioxidant, hypolipidemic and cytotoxic potential of Parmelia perlata were assessed. Methanolic extract of P. perlata was prepared and used in DPPH assay, total phenolic content and total antioxidant potential assays. In vitro anti-cholesterol and cytotoxic assay using HCT 116 cell lines were performed. The results revealed that P. perlata contain high phenolic contents and antioxidant potential. Simvastatin was used as standard drug and the extracts demonstrated 48% anti-cholesterol activity. MTT assay using colon cancer cell lines (HCT 116) produced dose dependent cytotoxic effect with an IC50 value of 202.1 µg ml-1.
VL  - 2
IS  - 6-1
ER  -

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Author Information

Hafizur Rahman

Department of Biotechnology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Vijaya B

Department of Biotechnology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Soumya Ghosh

Department of Microbiology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Geetika Pant

Department of Biotechnology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA
Sibi G

Department of Biotechnology, Indian Academy Degree College, Centre for Research and Post Graduate Studies, Bangalore, INDIA

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Plain Text BibTeX RIS

APA Style

Hafizur Rahman, Vijaya B, Soumya Ghosh, Geetika Pant, Sibi G. (2014). In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata. American Journal of Life Sciences, 2(6-1), 7-10. https://doi.org/10.11648/j.ajls.s.2014020601.12

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ACS Style

Hafizur Rahman; Vijaya B; Soumya Ghosh; Geetika Pant; Sibi G. In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata. Am. J. Life Sci. 2014, 2(6-1), 7-10. doi: 10.11648/j.ajls.s.2014020601.12

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AMA Style

Hafizur Rahman, Vijaya B, Soumya Ghosh, Geetika Pant, Sibi G. In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata. Am J Life Sci. 2014;2(6-1):7-10. doi: 10.11648/j.ajls.s.2014020601.12

Copy | Download

@article{10.11648/j.ajls.s.2014020601.12,
  author = {Hafizur Rahman and Vijaya B and Soumya Ghosh and Geetika Pant and Sibi G},
  title = {In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {6-1},
  pages = {7-10},
  doi = {10.11648/j.ajls.s.2014020601.12},
  url = {https://doi.org/10.11648/j.ajls.s.2014020601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2014020601.12},
  abstract = {New drug preparations of natural origin are in need due to the numerous side effects and resistance development through the continuous and uncontrolled use of synthetic drugs. Lichens are reported to have manifold biological activities and  in this study, antioxidant, hypolipidemic and cytotoxic potential of Parmelia perlata were assessed. Methanolic extract of P. perlata was prepared and used in DPPH assay, total phenolic content and total antioxidant potential assays. In vitro anti-cholesterol and cytotoxic assay using HCT 116 cell lines were performed. The results revealed that P. perlata contain high phenolic contents and antioxidant potential. Simvastatin was used as standard drug and the extracts demonstrated 48% anti-cholesterol activity. MTT assay using colon cancer cell lines (HCT 116) produced dose dependent cytotoxic effect with an IC50 value of 202.1 µg ml-1.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - In Vitro Studies on Antioxidant, Hypolipidemic and Cytotoxic Potential of Parmelia Perlata
AU  - Hafizur Rahman
AU  - Vijaya B
AU  - Soumya Ghosh
AU  - Geetika Pant
AU  - Sibi G
Y1  - 2014/08/24
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.s.2014020601.12
DO  - 10.11648/j.ajls.s.2014020601.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 7
EP  - 10
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.s.2014020601.12
AB  - New drug preparations of natural origin are in need due to the numerous side effects and resistance development through the continuous and uncontrolled use of synthetic drugs. Lichens are reported to have manifold biological activities and  in this study, antioxidant, hypolipidemic and cytotoxic potential of Parmelia perlata were assessed. Methanolic extract of P. perlata was prepared and used in DPPH assay, total phenolic content and total antioxidant potential assays. In vitro anti-cholesterol and cytotoxic assay using HCT 116 cell lines were performed. The results revealed that P. perlata contain high phenolic contents and antioxidant potential. Simvastatin was used as standard drug and the extracts demonstrated 48% anti-cholesterol activity. MTT assay using colon cancer cell lines (HCT 116) produced dose dependent cytotoxic effect with an IC50 value of 202.1 µg ml-1.
VL  - 2
IS  - 6-1
ER  -

Copy | Download