Periodontitis is an important complication in patients with diabetes mellitus. Chronic periodontitis is caused mostly by Porphyromonas gingivalis in Mexico, and by proinflammatory cytokines like IL-1, IL-6, TNFa, and IL-18. The aim of our study was to quantify IL-18 and to detect antibodies against P. gingivalis in gingival crevicular fluid of type 2 diabetes mellitus (T2DM) patients with chronic periodontal disease (PD). We studied a total of 40 patients divided in four groups: group 1 T2DM patients with PD, group 2 T2DM patients without PD, group 3 non-diabetic patients with PD, and a control group of healthy individuals. IL-18 was quantified using a commercial ELISA kit. Detection of IgG and IgM antibodies in gingival crevicular fluid was carried out through ELISA using P. gingivalis semi-purified proteins. Our results showed T2DM patients with chronic PD had low levels of IL-18, whereas non-diabetic patients with chronic PD had the highest. IgM antibodies were detected in diabetic and non-diabetic with chronic PD. IgG antibodies were detected in patients with T2DM and chronic PD, non-diabetic patients with chronic PD, and healthy individuals. In conclusion, IL-18 was detected in low concentrations in gingival crevicular fluid of patients with T2DM and chronic PD. This could be due to a dysregulation of immune-inflammatory responses secondary to diabetes, or by the presence of IL-18 binding protein in gingival fluid. Low levels of IgG antibodies in T2DM patients with PD could be due to the formation of immune complexes, which would explain the greater periodontal damage these patients present.
| Published in | International Journal of Immunology (Volume 4, Issue 4) |
| DOI | 10.11648/j.iji.20160404.11 |
| Page(s) | 20-26 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Antibodies, Porphyromonas gingivalis, Cytokines, Type 2 Diabetes, Periodontitis
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APA Style
Romero-Herrera Coralia, De La Rosa-Ramírez Antonio Manuel, Rosas-Taraco Adrián Geovanni, Limón-Flores Alberto Yairh, Arce-Mendoza Alma Yolanda. (2016). IL-18 and Anti- P. gingivalis Antibodies in Patients with Diabetes and Chronic Periodontitis. International Journal of Immunology, 4(4), 20-26. https://doi.org/10.11648/j.iji.20160404.11
ACS Style
Romero-Herrera Coralia; De La Rosa-Ramírez Antonio Manuel; Rosas-Taraco Adrián Geovanni; Limón-Flores Alberto Yairh; Arce-Mendoza Alma Yolanda. IL-18 and Anti- P. gingivalis Antibodies in Patients with Diabetes and Chronic Periodontitis. Int. J. Immunol. 2016, 4(4), 20-26. doi: 10.11648/j.iji.20160404.11
AMA Style
Romero-Herrera Coralia, De La Rosa-Ramírez Antonio Manuel, Rosas-Taraco Adrián Geovanni, Limón-Flores Alberto Yairh, Arce-Mendoza Alma Yolanda. IL-18 and Anti- P. gingivalis Antibodies in Patients with Diabetes and Chronic Periodontitis. Int J Immunol. 2016;4(4):20-26. doi: 10.11648/j.iji.20160404.11
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Download@article{10.11648/j.iji.20160404.11,
author = {Romero-Herrera Coralia and De La Rosa-Ramírez Antonio Manuel and Rosas-Taraco Adrián Geovanni and Limón-Flores Alberto Yairh and Arce-Mendoza Alma Yolanda},
title = {IL-18 and Anti- P. gingivalis Antibodies in Patients with Diabetes and Chronic Periodontitis},
journal = {International Journal of Immunology},
volume = {4},
number = {4},
pages = {20-26},
doi = {10.11648/j.iji.20160404.11},
url = {https://doi.org/10.11648/j.iji.20160404.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20160404.11},
abstract = {Periodontitis is an important complication in patients with diabetes mellitus. Chronic periodontitis is caused mostly by Porphyromonas gingivalis in Mexico, and by proinflammatory cytokines like IL-1, IL-6, TNFa, and IL-18. The aim of our study was to quantify IL-18 and to detect antibodies against P. gingivalis in gingival crevicular fluid of type 2 diabetes mellitus (T2DM) patients with chronic periodontal disease (PD). We studied a total of 40 patients divided in four groups: group 1 T2DM patients with PD, group 2 T2DM patients without PD, group 3 non-diabetic patients with PD, and a control group of healthy individuals. IL-18 was quantified using a commercial ELISA kit. Detection of IgG and IgM antibodies in gingival crevicular fluid was carried out through ELISA using P. gingivalis semi-purified proteins. Our results showed T2DM patients with chronic PD had low levels of IL-18, whereas non-diabetic patients with chronic PD had the highest. IgM antibodies were detected in diabetic and non-diabetic with chronic PD. IgG antibodies were detected in patients with T2DM and chronic PD, non-diabetic patients with chronic PD, and healthy individuals. In conclusion, IL-18 was detected in low concentrations in gingival crevicular fluid of patients with T2DM and chronic PD. This could be due to a dysregulation of immune-inflammatory responses secondary to diabetes, or by the presence of IL-18 binding protein in gingival fluid. Low levels of IgG antibodies in T2DM patients with PD could be due to the formation of immune complexes, which would explain the greater periodontal damage these patients present.},
year = {2016}
}
TY - JOUR T1 - IL-18 and Anti- P. gingivalis Antibodies in Patients with Diabetes and Chronic Periodontitis AU - Romero-Herrera Coralia AU - De La Rosa-Ramírez Antonio Manuel AU - Rosas-Taraco Adrián Geovanni AU - Limón-Flores Alberto Yairh AU - Arce-Mendoza Alma Yolanda Y1 - 2016/11/01 PY - 2016 N1 - https://doi.org/10.11648/j.iji.20160404.11 DO - 10.11648/j.iji.20160404.11 T2 - International Journal of Immunology JF - International Journal of Immunology JO - International Journal of Immunology SP - 20 EP - 26 PB - Science Publishing Group SN - 2329-1753 UR - https://doi.org/10.11648/j.iji.20160404.11 AB - Periodontitis is an important complication in patients with diabetes mellitus. Chronic periodontitis is caused mostly by Porphyromonas gingivalis in Mexico, and by proinflammatory cytokines like IL-1, IL-6, TNFa, and IL-18. The aim of our study was to quantify IL-18 and to detect antibodies against P. gingivalis in gingival crevicular fluid of type 2 diabetes mellitus (T2DM) patients with chronic periodontal disease (PD). We studied a total of 40 patients divided in four groups: group 1 T2DM patients with PD, group 2 T2DM patients without PD, group 3 non-diabetic patients with PD, and a control group of healthy individuals. IL-18 was quantified using a commercial ELISA kit. Detection of IgG and IgM antibodies in gingival crevicular fluid was carried out through ELISA using P. gingivalis semi-purified proteins. Our results showed T2DM patients with chronic PD had low levels of IL-18, whereas non-diabetic patients with chronic PD had the highest. IgM antibodies were detected in diabetic and non-diabetic with chronic PD. IgG antibodies were detected in patients with T2DM and chronic PD, non-diabetic patients with chronic PD, and healthy individuals. In conclusion, IL-18 was detected in low concentrations in gingival crevicular fluid of patients with T2DM and chronic PD. This could be due to a dysregulation of immune-inflammatory responses secondary to diabetes, or by the presence of IL-18 binding protein in gingival fluid. Low levels of IgG antibodies in T2DM patients with PD could be due to the formation of immune complexes, which would explain the greater periodontal damage these patients present. VL - 4 IS - 4 ER -
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