Ameliorative Effect of Ascorbic Acid and <i>Hibiscus sabdariffa</i> on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats

Umosen Angela Jecinta; Onyeyili Patrick Azubuike; Adenkola Adeshina Yahaya; Rabo Jude Samani

doi:doi:10.11648/j.jher.20180401.12

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Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats

Umosen Angela Jecinta, Onyeyili Patrick Azubuike, Adenkola Adeshina Yahaya, Rabo Jude Samani

Published in Journal of Health and Environmental Research (Volume 4, Issue 1)

Received: 9 January 2018 Accepted: 1 February 2018 Published: 26 February 2018

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Abstract

The present study evaluated the toxic effects of exposure of cement kiln dust, an environmental toxicant on erythrocyte fragility, lipid peroxidation and the ameliorative effect of ascorbic acid and Hibiscus sabdariffa in wistar rats. Thirty six adult male wistar rats divided into six groups of six rats each were used for the study. Rats in group I, II, III and V were administered distilled water (2 ml), ascorbic acid (100 mg/kg), cement kiln dust (250 mg/kg) and Hibiscus sabdariffa (100 mg/kg) respectively. Rats in group IV and VI were treated with ascorbic acid (100 mg/kg) and Hibiscus sabdariffa (100 mg/kg) respectively 30 minutes before administration of cement kiln dust (250 mg/kg). The treatments were administered by gavage once daily for 90 days. The animals were sacrificed at the end of the treatment period and blood samples collected were analyzed for erythrocyte osmotic fragility and malondialdehyde concentrations using standard methods. The study recorded a higher (p < 0.05) significant malondialdehyde concentration with values of 3.3 ± 0.1 µmol/l in group III (cement kiln dust treated group), while the lowest significant (p < 0.05) was obtained in group II (ascorbic acid group) with values of 0.6 ± 0.1 µmol/l. However the groups administered ascorbic acid and Hibiscus sabdariffa prior to cement kiln dust had significant (p < 0.05) lower levels when compared with group III (cement kiln dust treated group). There was significant (p < 0.05) increase in erythrocyte osmotic fragility in the cement kiln dust treated group, which decreased in the groups co-administered cement kiln dust and ascorbic acid or Hibiscus sabdariffa. The study concluded that prolonged exposure to cement kiln dust caused an increase in malondialdehyde concentration and high erythrocyte osmotic fragility, thus increased hemolysis probably due to increased lipid peroxidation, and administration of ascorbic acid and Hibiscus sabdariffa ameliorated these alterations.

Published in	Journal of Health and Environmental Research (Volume 4, Issue 1)
DOI	10.11648/j.jher.20180401.12
Page(s)	10-15
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Cement kiln Dust, Lipid Peroxidation, Ascorbic Acid, Hibiscus sabdariffa

References

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Umosen Angela Jecinta, Onyeyili Patrick Azubuike, Adenkola Adeshina Yahaya, Rabo Jude Samani. (2018). Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats. Journal of Health and Environmental Research, 4(1), 10-15. https://doi.org/10.11648/j.jher.20180401.12

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ACS Style

Umosen Angela Jecinta; Onyeyili Patrick Azubuike; Adenkola Adeshina Yahaya; Rabo Jude Samani. Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats. J. Health Environ. Res. 2018, 4(1), 10-15. doi: 10.11648/j.jher.20180401.12

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AMA Style

Umosen Angela Jecinta, Onyeyili Patrick Azubuike, Adenkola Adeshina Yahaya, Rabo Jude Samani. Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats. J Health Environ Res. 2018;4(1):10-15. doi: 10.11648/j.jher.20180401.12

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@article{10.11648/j.jher.20180401.12,
  author = {Umosen Angela Jecinta and Onyeyili Patrick Azubuike and Adenkola Adeshina Yahaya and Rabo Jude Samani},
  title = {Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats},
  journal = {Journal of Health and Environmental Research},
  volume = {4},
  number = {1},
  pages = {10-15},
  doi = {10.11648/j.jher.20180401.12},
  url = {https://doi.org/10.11648/j.jher.20180401.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jher.20180401.12},
  abstract = {The present study evaluated the toxic effects of exposure of cement kiln dust, an environmental toxicant on erythrocyte fragility, lipid peroxidation and the ameliorative effect of ascorbic acid and Hibiscus sabdariffa in wistar rats. Thirty six adult male wistar rats divided into six groups of six rats each were used for the study. Rats in group I, II, III and V were administered distilled water (2 ml), ascorbic acid (100 mg/kg), cement kiln dust (250 mg/kg) and Hibiscus sabdariffa (100 mg/kg) respectively. Rats in group IV and VI were treated with ascorbic acid (100 mg/kg) and Hibiscus sabdariffa (100 mg/kg) respectively 30 minutes before administration of cement kiln dust (250 mg/kg). The treatments were administered by gavage once daily for 90 days. The animals were sacrificed at the end of the treatment period and blood samples collected were analyzed for erythrocyte osmotic fragility and malondialdehyde concentrations using standard methods. The study recorded a higher (p Hibiscus sabdariffa prior to cement kiln dust had significant (p Hibiscus sabdariffa. The study concluded that prolonged exposure to cement kiln dust caused an increase in malondialdehyde concentration and high erythrocyte osmotic fragility, thus increased hemolysis probably due to increased lipid peroxidation, and administration of ascorbic acid and Hibiscus sabdariffa ameliorated these alterations.},
 year = {2018}
}

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TY  - JOUR
T1  - Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats
AU  - Umosen Angela Jecinta
AU  - Onyeyili Patrick Azubuike
AU  - Adenkola Adeshina Yahaya
AU  - Rabo Jude Samani
Y1  - 2018/02/26
PY  - 2018
N1  - https://doi.org/10.11648/j.jher.20180401.12
DO  - 10.11648/j.jher.20180401.12
T2  - Journal of Health and Environmental Research
JF  - Journal of Health and Environmental Research
JO  - Journal of Health and Environmental Research
SP  - 10
EP  - 15
PB  - Science Publishing Group
SN  - 2472-3592
UR  - https://doi.org/10.11648/j.jher.20180401.12
AB  - The present study evaluated the toxic effects of exposure of cement kiln dust, an environmental toxicant on erythrocyte fragility, lipid peroxidation and the ameliorative effect of ascorbic acid and Hibiscus sabdariffa in wistar rats. Thirty six adult male wistar rats divided into six groups of six rats each were used for the study. Rats in group I, II, III and V were administered distilled water (2 ml), ascorbic acid (100 mg/kg), cement kiln dust (250 mg/kg) and Hibiscus sabdariffa (100 mg/kg) respectively. Rats in group IV and VI were treated with ascorbic acid (100 mg/kg) and Hibiscus sabdariffa (100 mg/kg) respectively 30 minutes before administration of cement kiln dust (250 mg/kg). The treatments were administered by gavage once daily for 90 days. The animals were sacrificed at the end of the treatment period and blood samples collected were analyzed for erythrocyte osmotic fragility and malondialdehyde concentrations using standard methods. The study recorded a higher (p Hibiscus sabdariffa prior to cement kiln dust had significant (p Hibiscus sabdariffa. The study concluded that prolonged exposure to cement kiln dust caused an increase in malondialdehyde concentration and high erythrocyte osmotic fragility, thus increased hemolysis probably due to increased lipid peroxidation, and administration of ascorbic acid and Hibiscus sabdariffa ameliorated these alterations.
VL  - 4
IS  - 1
ER  -

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Author Information

Umosen Angela Jecinta

Department of Veterinary Physiology, Pharmacology and Biochemistry, University of Agriculture, Makurdi, Nigeria
Onyeyili Patrick Azubuike

Department of Veterinary Physiology, Pharmacology and Biochemistry, University of Agriculture, Makurdi, Nigeria
Adenkola Adeshina Yahaya

Department of Veterinary Physiology, Pharmacology and Biochemistry, University of Agriculture, Makurdi, Nigeria
Rabo Jude Samani

Department of Pathology and Microbiology, University of Agriculture, Makurdi, Nigeria

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Umosen Angela Jecinta, Onyeyili Patrick Azubuike, Adenkola Adeshina Yahaya, Rabo Jude Samani. (2018). Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats. Journal of Health and Environmental Research, 4(1), 10-15. https://doi.org/10.11648/j.jher.20180401.12

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ACS Style

Umosen Angela Jecinta; Onyeyili Patrick Azubuike; Adenkola Adeshina Yahaya; Rabo Jude Samani. Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats. J. Health Environ. Res. 2018, 4(1), 10-15. doi: 10.11648/j.jher.20180401.12

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AMA Style

Umosen Angela Jecinta, Onyeyili Patrick Azubuike, Adenkola Adeshina Yahaya, Rabo Jude Samani. Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats. J Health Environ Res. 2018;4(1):10-15. doi: 10.11648/j.jher.20180401.12

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@article{10.11648/j.jher.20180401.12,
  author = {Umosen Angela Jecinta and Onyeyili Patrick Azubuike and Adenkola Adeshina Yahaya and Rabo Jude Samani},
  title = {Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats},
  journal = {Journal of Health and Environmental Research},
  volume = {4},
  number = {1},
  pages = {10-15},
  doi = {10.11648/j.jher.20180401.12},
  url = {https://doi.org/10.11648/j.jher.20180401.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jher.20180401.12},
  abstract = {The present study evaluated the toxic effects of exposure of cement kiln dust, an environmental toxicant on erythrocyte fragility, lipid peroxidation and the ameliorative effect of ascorbic acid and Hibiscus sabdariffa in wistar rats. Thirty six adult male wistar rats divided into six groups of six rats each were used for the study. Rats in group I, II, III and V were administered distilled water (2 ml), ascorbic acid (100 mg/kg), cement kiln dust (250 mg/kg) and Hibiscus sabdariffa (100 mg/kg) respectively. Rats in group IV and VI were treated with ascorbic acid (100 mg/kg) and Hibiscus sabdariffa (100 mg/kg) respectively 30 minutes before administration of cement kiln dust (250 mg/kg). The treatments were administered by gavage once daily for 90 days. The animals were sacrificed at the end of the treatment period and blood samples collected were analyzed for erythrocyte osmotic fragility and malondialdehyde concentrations using standard methods. The study recorded a higher (p Hibiscus sabdariffa prior to cement kiln dust had significant (p Hibiscus sabdariffa. The study concluded that prolonged exposure to cement kiln dust caused an increase in malondialdehyde concentration and high erythrocyte osmotic fragility, thus increased hemolysis probably due to increased lipid peroxidation, and administration of ascorbic acid and Hibiscus sabdariffa ameliorated these alterations.},
 year = {2018}
}

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TY  - JOUR
T1  - Ameliorative Effect of Ascorbic Acid and Hibiscus sabdariffa on Cement Kiln Dust-induced Erythrocyte Osmotic Fragility and Lipid Peroxidation in Wistar Rats
AU  - Umosen Angela Jecinta
AU  - Onyeyili Patrick Azubuike
AU  - Adenkola Adeshina Yahaya
AU  - Rabo Jude Samani
Y1  - 2018/02/26
PY  - 2018
N1  - https://doi.org/10.11648/j.jher.20180401.12
DO  - 10.11648/j.jher.20180401.12
T2  - Journal of Health and Environmental Research
JF  - Journal of Health and Environmental Research
JO  - Journal of Health and Environmental Research
SP  - 10
EP  - 15
PB  - Science Publishing Group
SN  - 2472-3592
UR  - https://doi.org/10.11648/j.jher.20180401.12
AB  - The present study evaluated the toxic effects of exposure of cement kiln dust, an environmental toxicant on erythrocyte fragility, lipid peroxidation and the ameliorative effect of ascorbic acid and Hibiscus sabdariffa in wistar rats. Thirty six adult male wistar rats divided into six groups of six rats each were used for the study. Rats in group I, II, III and V were administered distilled water (2 ml), ascorbic acid (100 mg/kg), cement kiln dust (250 mg/kg) and Hibiscus sabdariffa (100 mg/kg) respectively. Rats in group IV and VI were treated with ascorbic acid (100 mg/kg) and Hibiscus sabdariffa (100 mg/kg) respectively 30 minutes before administration of cement kiln dust (250 mg/kg). The treatments were administered by gavage once daily for 90 days. The animals were sacrificed at the end of the treatment period and blood samples collected were analyzed for erythrocyte osmotic fragility and malondialdehyde concentrations using standard methods. The study recorded a higher (p Hibiscus sabdariffa prior to cement kiln dust had significant (p Hibiscus sabdariffa. The study concluded that prolonged exposure to cement kiln dust caused an increase in malondialdehyde concentration and high erythrocyte osmotic fragility, thus increased hemolysis probably due to increased lipid peroxidation, and administration of ascorbic acid and Hibiscus sabdariffa ameliorated these alterations.
VL  - 4
IS  - 1
ER  -

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