Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.

Chinelo Anthonia Ezeabara; Ikeh; Chigozie Faith; Chinyere Veronica Ilodibia; Bibian Okwuchukwu Aziagba; Ogochukwu Esther Okanume; Mbaekwe; Ebenezer Ike

doi:doi:10.11648/j.jps.20140206.15

Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.

Chinelo Anthonia Ezeabara, Ikeh, Chigozie Faith, Chinyere Veronica Ilodibia, Bibian Okwuchukwu Aziagba, Ogochukwu Esther Okanume, Mbaekwe, Ebenezer Ike

Published in Journal of Plant Sciences (Volume 2, Issue 6)

Received: 18 November 2014 Accepted: 28 November 2014 Published: 15 December 2014

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Abstract

Aqueous and ethanol extracts of leaf, stem and root of Portulaca oleracea L. were analyzed for phytochemical constituents and compared, whereas only aqueous extract was used for proximate and mineral composition determinations. There were presence of alkaloid, flavonoid, phenol, saponin, sterol, tannin and terpenoid in the leaf, stem and root of both extracts. Alkaloid and saponin have highest concentrations in both aqueous and ethanol extracts. High concentrations of calcium, magnesium, phosphorus and potassium were present in the leaf, stem and root, whereas iron content was least in all the parts. Level of crude protein, crude fibre and fat were highest in the leaf while carbohydrate was highest in the stem and least in the leaf at p>0.05 level of significance. Generally, it was observed that various parts of Portulaca oleracea contained different concentrations of phytochemicals and nutrients. This work, therefore, confirmed the plant’s indigenous uses. In addition, it proposed extraction of the bioactive compounds and nutrients from them for manufacture of food supplements and development of new drugs. Ethanol extract of this plant is preferable for extraction of saponin, tannin and terpenoid whereas both aqueous and ethanol extracts would produce good level of alkaloid, flavonoid, phenol and sterol.

Published in	Journal of Plant Sciences (Volume 2, Issue 6)
DOI	10.11648/j.jps.20140206.15
Page(s)	293-298
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Portulaca oleracea, Aqueous extract, Ethanol extract, Leaf, Stem, Root, Alkaloid, Saponin, Potassium, Protein

References

[1]	L.W. Mitich, Common Purslane (Portulaca oleracea), Weed Technology, 1997; 11 (2): 394-397.
[2]	H.M. Burkill, The Useful Plants of West Tropical Africa, 2nd ed., Vol 1. Royal Botanic Garden, Kew.1985; pp. 960.
[3]	M. Iwu, Handbook of African Medicinal Plants, 2nd ed. CRC Press, Boca Raton. 1993; pp. 464.
[4]	Y.Y. Lim, E.P.L. Quah, Antioxidant properties of different cultivars of Portulaca oleracea. Food Chemistry, 2007; 103:734–740.
[5]	W. Richard, Invasive Species Specialist Group (ISSG), Global Invasive Species Data Base. Annals of Botany, 2005; 21: 287-314.
[6]	A.H. Beckett, J.B. Stenlake, Practical Pharmaceutical Chemistry: Part 1. Continuum International Publishing Group Ltd., London. 1988; pp. 235.
[7]	J.B. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, 3rd ed. Chapman and Hall, London. 1988; pp.302.
[8]	Association of Official Analytical Chemists, Official Methods of Analysis, 15th ed. International Association of Official Analytical Chemists, Washington D.C. 1990; pp. 409.
[9]	G.I. Onwuka, Food Analysis and Instrumentation: Theory and Practice. Naphthali Prints, a Division of HG Support Nigeria Ltd., Lagos. 2005; pp. 250.
[10]	F. Agha-Hosseini, K., Borhan-Mojabi, H.R. Monsef-Esfahani, I. Mirzaii-Dizgah, S. Etemad-Moghadam, A. Karagah, Efficacy of purslane in the treatment of oral lichen planus. Phytotherapy Research, 2010; 24 (2): 240–4.
[11]	D. Bensky, Chinese Herbal Medicine, Materia Medica. Eastland Press Inc., China. 2004; pp.1050.
[12]	J.C. Okafor, Tropical Plants in Health Care Delivery in Nigeria. Ibadan: Bookbuilders, 2013; pp. 188.
[13]	G.A. Aderinokun, J.O. Lawoyin, C.O. Onyeaso, Effect of two common Nigerian chewing sticks on gingival health and oral hygiene. Odonto-Stomatologie Tropicale, 1999; 22 (87): 13–18.
[14]	D. Herourat, R.S. Sangwin, M.A. Finiaux, B.S. Sangwan-Norrell, Variations in the leaf alkaloid content of androgenic diploid plants of Daturu innoxia Planta medical. Journal of Medicinal Plant Research, 1988; 54: 14-20.
[15]	O.V. Njoku, C. Obi, Phytochemical constituents of some selected medicinal plants. African Journal of Pure and Applied Chemistry, 2009; 3 (11):228-233.
[16]	Y. Yamamoto, R.B. Gaynor, Therapeutic potential of inhibition of the NF-B pathway in the treatment of inflammation and cancer. Journal of Clinical Investigation, 2001; 107 (2): 135–42.
[17]	G. Francis, Z. Kerem, H.P.S. Makkar, K. Becker, The biological action of saponins in animal systems: a review. British Journal of Nutrition, 2002; 88:587–605.
[18]	M.P. Argentieri, T.A. D ́Addobbo, A. Agostinelli, M. Jurzysta, P. Avato, Evaluation of nematicidal properties of saponins from Medicago species. European Journal of Plant Pathology, 2007; 120:189-197.
[19]	J. Shi, K. Arunasalam, D. Yeung, Y. Kakuda, G. Mittal, Y. Jiang, Saponins from edible legumes: Chemistry, processing and health benefits. Journal of Medical Food, 2004; 7:67-78.
[20]	S.G. Sparg, M.E. Light, J. van Staden, Biological activities and distribution of plant saponins. Journal of Ethno Pharmacology, 2004; 94:219–243.
[21]	K. Gauthaman, P.G. Adaikan, R.N. Prasad, Aphrodisiac properties of Tribulus terrestris extract (Protodioscin) in normal and castrated rats. Life Science, 2002; 71:1385– 96.
[22]	Guardian, Herbal cocktail better than Viagra in humans, say researchers, April 19, 2009; Lagos, Nigeria.
[23]	H.P.S. Makkar, T. Norvsambuu, S. Lkhagvatseren, K. Becker, Plant Secondary Metabolites in some Medicinal Plants of Mongolia Used for Enhancing Animal Health and Production. Tropicultura, 2009; 27(3):159-167.
[24]	K.R. Price, G.R. Fenwick, The Chemistry and Biological significance of saponins in foods and feeding stuffs. International Review in Food Science and Nutrition, 1990; 157:62.27.
[25]	M.J. Just, M.G. Recsio, R.M. Gner, M.J. Cuellar, S. Marez, A.R., Bilia, J. Rios, Anti-inflammatory activity of unusual lupane saponins from Buleurum fruiticescens. Planta Medica, 1998; 64(5):404-407.
[26]	C.A. Ezeabara, C.U. Okeke, B.O. Aziagba, C.V. Ilodibia, A.N. Emeka, Determination of saponin content of various parts of six Citrus species. International Research Journal of Pure and Applied Chemistry, 2014; 4(1):137-143
[27]	L. Lu, S.W. Liu, S.B. Jiang, S.G. Wu, Tannin inhibits HIV-1 entry by targeting gp41. Acta Pharmacology Sinica, 2004; 25 (2): 213-218.
[28]	F. Tian, B. Li, B. Ji, Antioxidant and antimicrobial activities of consecutive extracts from Galla chinensis: the polarity affects the bioactivities. Food Chemistry, 2009; 113 (1):173–179.
[29]	H. Kolodziej, A.F. Kiderlen, Anti-leshmanial activity and immune modulatory effects of tannin and related compounds on leshmania parasitized RAW 264.7 cells. Phytochemistry, 2005; 66 (17): 2056- 71.
[30]	S. Foster, J.A. Duke, A Field Guild to Medicinal Plants and Herbs, 2nd ed. Houghton Mifflin Company, Boston. 2000; pp. 1657.
[31]	A.P. Simopoulos, H.A. Norman, J.E. Gillaspy, J.A. Duke, Common purslane: a source of omega-3 fatty acids and antioxidants. Journal of the American College of Nutrition, 1992; 11(4):374-82.
[32]	S.C. Larsson, M.J. Virtanen, M. Mars, S. Männistö, P. Pietinen, D. Albanes, J. Virtamo, Magnesium, calcium, potassium, and sodium intakes and risk of stroke in male smokers. Archives of Internal Medicine, 2008; 168 (5): 459–65.
[33]	A.A. McDonough, M.T.X. Nguyen, How does potassium supplementation lower blood pressure? Renal Physiology, 2012; 302:F1124-5.
[34]	E.N. Taylor, T.T. Fung, G.C. Curhan, DASH-style diet associates with reduced risk for kidney stones. Journal of the American Society of Nephrology, 2009; 20:2253-2259.
[35]	P.K. Whelton, J. He, J.A. Cutler, F.L. Brancati, L.J. Appel, D. Follmann, M.J. Klag, Effects of oral potassium on blood pressure: meta-analysis of randomised controlled clinical trials. The Journal of the American Medical Association, 1997; 277:1624-32.

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Chinelo Anthonia Ezeabara, Ikeh, Chigozie Faith, Chinyere Veronica Ilodibia, Bibian Okwuchukwu Aziagba, et al. (2014). Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.. Journal of Plant Sciences, 2(6), 293-298. https://doi.org/10.11648/j.jps.20140206.15

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ACS Style

Chinelo Anthonia Ezeabara; Ikeh; Chigozie Faith; Chinyere Veronica Ilodibia; Bibian Okwuchukwu Aziagba, et al. Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.. J. Plant Sci. 2014, 2(6), 293-298. doi: 10.11648/j.jps.20140206.15

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AMA Style

Chinelo Anthonia Ezeabara, Ikeh, Chigozie Faith, Chinyere Veronica Ilodibia, Bibian Okwuchukwu Aziagba, et al. Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.. J Plant Sci. 2014;2(6):293-298. doi: 10.11648/j.jps.20140206.15

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@article{10.11648/j.jps.20140206.15,
  author = {Chinelo Anthonia Ezeabara and Ikeh and Chigozie Faith and Chinyere Veronica Ilodibia and Bibian Okwuchukwu Aziagba and Ogochukwu Esther Okanume and Mbaekwe and Ebenezer Ike},
  title = {Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.},
  journal = {Journal of Plant Sciences},
  volume = {2},
  number = {6},
  pages = {293-298},
  doi = {10.11648/j.jps.20140206.15},
  url = {https://doi.org/10.11648/j.jps.20140206.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20140206.15},
  abstract = {Aqueous and ethanol extracts of leaf, stem and root of Portulaca oleracea L. were analyzed for phytochemical constituents and compared, whereas only aqueous extract was used for proximate and mineral composition determinations. There were presence of alkaloid, flavonoid, phenol, saponin, sterol, tannin and terpenoid in the leaf, stem and root of both extracts. Alkaloid and saponin have highest concentrations in both aqueous and ethanol extracts. High concentrations of calcium, magnesium, phosphorus and potassium were present in the leaf, stem and root, whereas iron content was least in all the parts. Level of crude protein, crude fibre and fat were highest in the leaf while carbohydrate was highest in the stem and least in the leaf at p>0.05 level of significance. Generally, it was observed that various parts of Portulaca oleracea contained different concentrations of phytochemicals and nutrients. This work, therefore, confirmed the plant’s indigenous uses. In addition, it proposed extraction of the bioactive compounds and nutrients from them for manufacture of food supplements and development of new drugs. Ethanol extract of this plant is preferable for extraction of saponin, tannin and terpenoid whereas both aqueous and ethanol extracts would produce good level of alkaloid, flavonoid, phenol and sterol.},
 year = {2014}
}

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TY - JOUR
T1 - Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.
AU - Chinelo Anthonia Ezeabara
AU - Ikeh
AU - Chigozie Faith
AU - Chinyere Veronica Ilodibia
AU - Bibian Okwuchukwu Aziagba
AU - Ogochukwu Esther Okanume
AU - Mbaekwe
AU - Ebenezer Ike
Y1 - 2014/12/15
PY - 2014
N1 - https://doi.org/10.11648/j.jps.20140206.15
DO - 10.11648/j.jps.20140206.15
T2 - Journal of Plant Sciences
JF - Journal of Plant Sciences
JO - Journal of Plant Sciences
SP - 293
EP - 298
PB - Science Publishing Group
SN - 2331-0731
UR - https://doi.org/10.11648/j.jps.20140206.15
AB - Aqueous and ethanol extracts of leaf, stem and root of Portulaca oleracea L. were analyzed for phytochemical constituents and compared, whereas only aqueous extract was used for proximate and mineral composition determinations. There were presence of alkaloid, flavonoid, phenol, saponin, sterol, tannin and terpenoid in the leaf, stem and root of both extracts. Alkaloid and saponin have highest concentrations in both aqueous and ethanol extracts. High concentrations of calcium, magnesium, phosphorus and potassium were present in the leaf, stem and root, whereas iron content was least in all the parts. Level of crude protein, crude fibre and fat were highest in the leaf while carbohydrate was highest in the stem and least in the leaf at p>0.05 level of significance. Generally, it was observed that various parts of Portulaca oleracea contained different concentrations of phytochemicals and nutrients. This work, therefore, confirmed the plant’s indigenous uses. In addition, it proposed extraction of the bioactive compounds and nutrients from them for manufacture of food supplements and development of new drugs. Ethanol extract of this plant is preferable for extraction of saponin, tannin and terpenoid whereas both aqueous and ethanol extracts would produce good level of alkaloid, flavonoid, phenol and sterol.
VL - 2
IS - 6
ER -

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Author Information

Chinelo Anthonia Ezeabara

Department of Botany, Nnamdi Azikiwe University, P.M.B. 5025 Awka, Anambra State, Nigeria
Ikeh
Chigozie Faith
Chinyere Veronica Ilodibia

Department of Botany, Nnamdi Azikiwe University, P.M.B. 5025 Awka, Anambra State, Nigeria
Bibian Okwuchukwu Aziagba

Department of Botany, Nnamdi Azikiwe University, P.M.B. 5025 Awka, Anambra State, Nigeria
Ogochukwu Esther Okanume

Department of Plant Science and Technology, University of Jos, Plateau State, Nigeria
Mbaekwe
Ebenezer Ike

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Chinelo Anthonia Ezeabara, Ikeh, Chigozie Faith, Chinyere Veronica Ilodibia, Bibian Okwuchukwu Aziagba, et al. (2014). Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.. Journal of Plant Sciences, 2(6), 293-298. https://doi.org/10.11648/j.jps.20140206.15

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Chinelo Anthonia Ezeabara; Ikeh; Chigozie Faith; Chinyere Veronica Ilodibia; Bibian Okwuchukwu Aziagba, et al. Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.. J. Plant Sci. 2014, 2(6), 293-298. doi: 10.11648/j.jps.20140206.15

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AMA Style

Chinelo Anthonia Ezeabara, Ikeh, Chigozie Faith, Chinyere Veronica Ilodibia, Bibian Okwuchukwu Aziagba, et al. Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.. J Plant Sci. 2014;2(6):293-298. doi: 10.11648/j.jps.20140206.15

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@article{10.11648/j.jps.20140206.15,
  author = {Chinelo Anthonia Ezeabara and Ikeh and Chigozie Faith and Chinyere Veronica Ilodibia and Bibian Okwuchukwu Aziagba and Ogochukwu Esther Okanume and Mbaekwe and Ebenezer Ike},
  title = {Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L.},
  journal = {Journal of Plant Sciences},
  volume = {2},
  number = {6},
  pages = {293-298},
  doi = {10.11648/j.jps.20140206.15},
  url = {https://doi.org/10.11648/j.jps.20140206.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20140206.15},
  abstract = {Aqueous and ethanol extracts of leaf, stem and root of Portulaca oleracea L. were analyzed for phytochemical constituents and compared, whereas only aqueous extract was used for proximate and mineral composition determinations. There were presence of alkaloid, flavonoid, phenol, saponin, sterol, tannin and terpenoid in the leaf, stem and root of both extracts. Alkaloid and saponin have highest concentrations in both aqueous and ethanol extracts. High concentrations of calcium, magnesium, phosphorus and potassium were present in the leaf, stem and root, whereas iron content was least in all the parts. Level of crude protein, crude fibre and fat were highest in the leaf while carbohydrate was highest in the stem and least in the leaf at p>0.05 level of significance. Generally, it was observed that various parts of Portulaca oleracea contained different concentrations of phytochemicals and nutrients. This work, therefore, confirmed the plant’s indigenous uses. In addition, it proposed extraction of the bioactive compounds and nutrients from them for manufacture of food supplements and development of new drugs. Ethanol extract of this plant is preferable for extraction of saponin, tannin and terpenoid whereas both aqueous and ethanol extracts would produce good level of alkaloid, flavonoid, phenol and sterol.},
 year = {2014}
}

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