Plant tissue culture is the technique to culture plant cells or tissues under controlled aseptic conditions on a synthetic medium .It has value in basic research like cell biology, genetic transformation studies &biochemistry for the production of medicinally valuable secondary metabolites. Besides this also has commercial application. This review work outlines the work done on the tissue culture of Momordica charantia L. Momordica charantia L. commonly known as bitter melon/gourd, a member of Cucarbitaceae, is a slender, tendril climbing, annual vine. Bitter melon is a common food item of the tropics and is used for the treatment of cancer, diabetes, AIDS and many ailments. It is a potent hypoglycemic agent and its hypoglycemic actions for potential benefit in diabetes mellitus are possible due to at least three different groups of constituents in bitter melon. These include alkaloids, insulin like peptides, and a mixture of steroidal sapogenins known as charantin. Clinical studies with multiple controls have confirmed the benefit of bitter melon for diabetes. Alpha and beta momarcharin are two proteins found in bitter melon, which are known to inhibit the AIDS virus.
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Journal of Plant Sciences (Volume 3, Issue 1-1)
This article belongs to the Special Issue Medicinal Plants |
| DOI | 10.11648/j.jps.s.2015030101.14 |
| Page(s) | 24-32 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Tissue Culture, Momordica charantia, Hypoglycemic, In Vitro Regeneration
| [1] | E.F. George, M.A. Hall, G.J Klerk, “Plant propagation by tissue culture,” Springer Dordrecht.1, 2008,501. |
| [2] | R.Yadav ,P. Arora, D. Kumar ,N. Katyal, D ,dilbagi ,and A. Chaudhary , “High frequency direct plant regeneration from leaf, internode and root segments of Eastern Cottonwood,”Vol83pp76-89,2009. |
| [3] | P.Garg, P. Khatri, and D. Gandhi, “Plant tissue culture of Jatropa curcas L; A review,”Imperial Journal of Pharmacognosy & Natural Products1Vol1, pp6-13, 2011. |
| [4] | M.Georgiev, J. Weber, .and A. Maciuk, “Bioprocessing of Plant Cell Cultures for mass production of targeted compounds,” Applied Microbiology and Biotechnology, Vol83, pp809-823, 2009. |
| [5] | N. Sunderland, E. C. Cocking, “ Plant tissue culture in China-major changes ahead” Nature,Vol274,pp 643-644,1978. |
| [6] | P.J.Botino, “Methods in plant tissue culture. Kentec Educational Corporation, Kensington, Marylan,” 1981, pp72. |
| [7] | J.H.Dodds, and L.W. Roberts, “Experiments in plant tissue culture,” Cambridge University Press, New York, 1985,232. |
| [8] | H.E.Street, “Plant tissue and Cell Culture” Blackwell Scientific Publications, Oxford, 1973,320. |
| [9] | S.S.Bhojwani, and M.K. Razdan, “Plant Tissue Culture; Theory and Practice, revised edition Elsevier,” 1996,767. |
| [10] | L.Mineo, “Plant tissue culture techniques, Tested studies for laboratory teaching,” .CA Goldman, Eds11, 1990, 151-174. |
| [11] | I.P.Ting, “Plant Physiology,” Addison-Wesleyn Reading, Massachusetts, 1982, pp 642. |
| [12] | R.Barceto, S. Rasco-Gaunt, C. Thorpe, P. Lazzeri, “Transformation and gene expression advances in botanical research,” Biotechnology of Cereals. Academic Press, London, 34 2001pp, 59-126, 2001. |
| [13] | R.IBakare ,O.A. Mabagabeola ,A.L. Akinwande ,and O.W. Okunowo , “ Nutritional and chemical evaluation of Momordica charantia”J Med Plant ResearchVol4(21),pp2189-2193,2010. |
| [14] | G.Balasubramanian, M. Sarathi, S.R. Kumar, A.S. Hameed, “Screening the antiviral activity of Indian medicinal plants against white spot syndrome virus in shrimp”, Aquaculture Vol 263, pp15-19, 2007. |
| [15] | E.Basch ,S. Gabardi ,and C. Ulbaricht, “ .Bitter melon (Momordica charantia): A review of efficacy and safety” Am J. Health Syst Pharm.Vol60,pp 356-359,2003. |
| [16] | P.Khanna,and S.Mohan , “ Isolation and identification of diosgenin and sterols from fruits and in vitro cultures of Momordica charantia L.”, Indian J. Exp. Biol,Vol, 11pp 110,1973. |
| [17] | A.Raman,and C. Lau , “ Anti-diabetic properties and phytochemistry of Momordica charanantia L. (Cucurbitaceae)”, Phytomed,Vol 2,pp 349-62,1996. |
| [18] | A.Singh,S.P. Singh, R.Bennezai, “Momordica charantia(Bitter Gourd)peel,pulp,seed and whole fruit extract inhibits mouse stem papillomagenesis.Toxicol. Lett.Vol(94)pp37-46,1998. |
| [19] | L.Taylor , “ Tropical plant databases. Available at: http://www.rain-tree.com /plants. htm. Accessed” 08/10/04,2004. |
| [20] | Q.C.Zhang, “ Preliminary report on the use of M.charantia extracted by HIV patients”, J naturopath medicineVol3,pp 65-69,1992. |
| [21] | Y. Manye, M.Zhao, L.Lan, F.Chen, “Establishment of in vitro regeneration system of bitter melon(Momordica charantia L.),Vol(10)pp44-48,2004. |
| [22] | G.L.Wang,H.Y. Fan ,R.Y Lin ,X.T. Li , “ Establishment of in vitro Regeneration System of Momordica charantia L. with Different Genotype,” J. An Hui Agric. Sci., Vol 36(28)pp 12125-12127, 2008. |
| [23] | A.Huda,andBSikdar, “. In vitro Plant production through apical meristem culture of Bitter gourd(Momorica charantia L.)”Vol.16(1)pp31-36,2006 |
| [24] | T.Murashige ,F. Skoog , “ A revised medium for rapid growth and bioassays with tobacco tissue cultures”Physiol. Plant. Vol(15)pp473-497,1962. |
| [25] | S.Malik ,M. Zia ,R. Rehman, and, F. Chaudhary , “ In vitro plant regeneration from direct and indirect organogenesis of Momordica charantia,” Pak. J. Biol. Sci., Vol 10pp 4118-4122,2007. |
| [26] | .M.Thiruvengadam ,K.T. Rekha ,and N. Jayabalan, “ An efficient in vitro propagation of Momordica dioica Roxb. ex. Willd,” Philipp Agric. Scientist, Vol 89pp165-171,2006. |
| [27] | A.J.W.Berg, X.P. Zhang, and B.B. Rhodes, “ Micro Propagation of Citrullus lanatus (Thumb.) Matsum and Nakai (Water Melon),” in Biotechnology in Agriculture and Forestry. High-Tech and Micro Propagation., Bajaj, V.Y.P.S. (Ed.), Vol. 39. Springer-Verlag, Berlin Heidelberg,1997. |
| [28] | A. Nabi, M. M. Rashid, M. Al-Amin, M. G. Rasul, “Organogenesis in teasle gourd (Momordica dioica Roxb.),”. Plant Tissue Cult., Vol 12 pp 173-180,2002. |
| [29] | R.L.M.Pierik, “In vitro Propagation of Higher Plants,” Martinus Nizhoof Publisher, Boston,1987. |
| [30] | M. Agarwal, and R. Kamal, “In vitro clonal propagation of Momordica charantia L. Indian J. Biotechnol., Vol3pp 426-430,2004. |
| [31] | R. S. Sultana, and M. A. Bari Miah, “(2003). In vitro propagation of karalla (Momordica charantia L),” J. Biol. Sci.,Vol 3pp 1134-1139,2003. |
| [32] | A.Hoque, R. Islam, and O.I Joarder, “In vitro plantlets differentiation in kakrol (Momordica dioica Roxb.)”. Plant Tissue Cult., Vol 5, pp119-124,1995. |
| [33] | M.Thiruvengadam ,K.T. Rekha ,C.H. Yang ,N. Jayabalan ,and M.Chang, “ High frequency shoot regeneration from leaf explants through organogenesis in bitter melon(Momordica charantia L.”)Plant Biotechnology Reports Vol4(4)pp321-328,2010. |
| [34] | O. L. Gamborg, R. A. Miller, and, K. Ojima, “Nutrients requirements of suspension cultures of soybean root cells,” Exp. Cell Res., Vol 50pp 151-158, 1968. |
| [35] | Y.Tang,J..,Liu ,X. Li .J. Li, and H. Li , “Promote Adventitious buds induction from stem segments of Bitter melon(Momordica charantia L.),”Journal of Agricultural Science,Vol3(2),2011. |
| [36] | M. Thiruvengadam ,N. Praveen, K.M.Maria ,and III –Min Chug, “In vitro regeneration from intermodal explants of bitter melon(Momordica charantia L.)via indirect organogenesis ,African J. of Biotechnology”Vol11(32),pp8218-8224,2012. |
| [37] | N.K.Devendra ,B. Subhash ,Y.N. Seetharam,“Callus growth and plant regeneration in Momordica dioica (Roxb.) Wild. Cucurbitaceae,” Am. Eurasian J. Sustain Agric., Vol 3,pp 743-748,2009. |
| [38] | N.Selvaraj, A. Vasudevan, M. Manickavasagam, and, A. Ganapathi, “In vitro organogenesis and plant formation in cucumber. Biol. Plant. Vol 50,pp 123-126,2006. |
| [39] | L.W.Handley,and O.L. Chambliss, “ In vitro propagation of Cucumis sativus L. Hort. Sci., ”Vol 14pp 22-23,1979. |
| [40] | Z. K. Punja, N. Abbas, C.G Sarmento, and F. A. Tang, “Regeneration of Cucumis sativus vars, sativus and hardwickii, C. melo and C. metuliferus from explants through somatic embryogenesis and organogenesis,”. 102,1990. |
| [41] | S.H.Seo ,D.G. Bai ,and H.Y. Park, “High frequency shoot regeneration from leaf explants of cucumber,” J. Plant Biotechnol., Vol 2pp 51-54,2000. |
| [42] | N.Selvaraj ,A. Vasudevan ,M. Manickavasagam ,S. Kasthurirengan,and,A. Ganapathi , “ High frequency shoot regeneration from cotyledon explants of cucumber via organogenesis,”. Sci. Hortic.,Vol 112pp 2-8,2007. |
| [43] | Savitha ,T. Shasthree , Sudhakar, B. Mallaiah , “ High frequency of plantlet regeneration and multiple shoot induction from leaf and stem explant of Citrullus colosynthis (L.) Schrad, an endangered medicinal cucurbit” Int. J. Pharma Biol. Sci.Vol 6(2)pp 1-8,2010. |
| [44] | S.P.Pal ,I. Alam ,M. Anisuzzaman ,K.K. Sarker ,S.A. Sharmin ,and M.F. Alam , “Indirect organogenesis in summer squash (Cucurbita pepo L.),” Turk. J. Agric. Forest. Vol 31pp 63-70, 2007. |
| [45] | P.Baskaran, P. Velayutham, N. Jayabalan, “In vitro regeneration of Melothria maderaspatana via indirect organogenesis. In Vitro Cell Dev. Biol. Plant., Vol 45pp 407-413, 2009. |
| [46] | A.Vasudevan ,N. Selvaraj ,A. Ganapathi ,S. Kasthurirengan ,V. Ramesh Anbazhagan, and M.Manickavasagam, “ Glutamine: a suitable nitrogen source for enhanced shoot multiplication in cucumber (Cucumis sativus L.),” Biol. Plant.,Vol 48pp 125-128,2004. |
| [47] | R.D.Locy,andT.C. Wehner , “ Cucumber shoot tip growth on 9 nitrogen sources in in vitro culture,” Cucurbits Genet. Coop. Rep.,Vol 5pp 10-11,1982. |
| [48] | S.Malepszy,and,A. Nadolska-Orezyk , “ In vitro culture of Cucumis sativus. : Regeneration of plantlets from callus formed by leaf explants. Z. Pflanzen physiol., Vol 111pp 273-276,1983. |
| [49] | J.H.W.Bergervoet ,F. Van der Mark ,and J.B.M. Custers, , “ Organogenesis versus embryogenesis from long-term suspension cultures of cucumber (Cucumis sativus L.),” Plant Cell Rep., Vol 8pp 116-119,1989. |
| [50] | A.J.Trulson ,and E.A. Shahin, “ In vitro plant generation in the genus Cucumis ,” Plant Sci.Vol 47 pp 35-43,1986. |
| [51] | A.Hoque,R.Islam,and O.I. Jorader, “In vitro plantlets differentiation in kakrol(Momordica dioica Roxb.)”,Plant tissue cult., Vol 5,pp119-125,1995. |
| [52] | T.D.Thomas, and K.R. Sreejesh, “Callus induction and plant regeneration from cotyledonary explants of ash gourd (Benincasa hispida L.),” Sci. Hortic., Vol 100pp 359-367, 2004. |
| [53] | K.Kathiravan ,G. Vengedesan ,S. Singer ,B. Steinitz ,H. Paris ,and V. Gaba , “ Adventitious regeneration in vitro occurs across a wide spectrum of squash (Cucurbita pepo) genotypes,”. Plant Cell Tissue Organ Cult., Vol 85,pp 285-295,2006. |
| [54] | J.S.Han ,D.G. Oh ,I.G. Mok ,H.G. Park ,and C.K. Kim , ”Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.),”. Plant Cell Rep. 23: 291-296,2004. |
| [55] | M.Chao,Yi.Tang ,Li. Xiaomei Li., wang,and Li. Xuanxiu, “In vitro induction of multiple buds from cotyledonary nodes of balasam(Momordica charantia L.)”African J. Biotechnology, Vol11(3)pp3106-3115,2012. |
| [56] | Y.F.Zhang, J. H Zhou, T. Wu, J.S. Cao, “Shoot regeneration and the relationship between organogenic capacity and endogenous hormonal contents in pumpkin,” Plant Cell, Tissue Org. Cult., Vol 93(3) pp 323-331,2008. |
| [57] | SV. O. Ntui, G. Thirukkumaran, and S. lioka, M. Mii, “Efficient plant regeneration via organogenesis in “Egusi” melon (Colocynthis citrullus L.),”. Sci. Hortic.,Vol 119pp 397-402, 2009. |
| [58] | Y. Z. Lin, H. Y. Luo ,Z.Z. Zhang , “ Induction of multiple buds from cotyledonary nodes of Balsam pear (Momordica charantia L.),” Chin. J. Trop Crops, Vol 27(2),pp 60-63,2006. |
| [59] | J.Li, H.X. Li, M. Li, “The System of In Vitro Culture and Shoot Regeneration from Cotyledon of Balsam Pear of Cuifei.,” Northern Hort., Vol 10pp 181-183,2007. |
| [60] | T.C. Wehner, and R.D, Locy, “In vitro adventitious shoot and root formation of cultivars and lines of Cucumis sativus L.,” Hort. Sci.Vol 16(6) pp 759-760, 1981. |
| [61] | Al Munsu MAZ, M. S. Haque, K. M. Nasiruddin, and M.S. Hossain, “In Vitro propagation of bitter gourd (Momordica charantia L.) from nodal and root segment. Plant Tissue Cult. Biotechnol., ”Vol 19(1),pp 45-52,2009. |
| [62] | R L.Y.Song, and F. Gao, “Changes of endogenous hormones in Momordica charantia L. during in vitro Culture,” Chinese Bull. BotVol 23(2)pp 192-196,2006. |
| [63] | J.H.Jeong ,H.N. Murthy ,K.Y Paek , “ High frequency adventitious shoots induction and plant regeneration from leaves of statice,” Plant Cell, Tissue Org. Cult., Vol 65(2)pp 123-128,2001. |
| [64] | S.Wang, L. Tang, F.chen, “In vitro flowering of bitter melon, ”.Plant Cell Reports, Vol 20(5) pp 393-397,2001. |
| [65] | J. Mishra, K. L. Tiwari, and S. K. Jadhav, “Micro propagation of Momordica charantia L., ”International J. of Biological and Health Sciences,”Vol1(1)pp25-31,2012. |
| [66] | M.. Thiruvengadam, S. Varisai Mohamed, C.H. Yang, N. Jayabalan, and M. Chang M, “Development of an embryogenic suspension culture of Bitter melon (Momordica charantia L.)” Follow Scientia Horticultuare Vol109 (2), 2006. |
| [67] | M..Thiruvengadam, N. Praveen, K.M. Maria Jin, Ye-Sul Yang, Seung-Hyun Kim, and III –Min Chung, “Plant cell, tissue and organ culture,Vol118(3),pp545-557,2014. |
APA Style
Mala Agarwal. (2014). Tissue Culture of Momordica charantia L.: A Review. Journal of Plant Sciences, 3(1-1), 24-32. https://doi.org/10.11648/j.jps.s.2015030101.14
ACS Style
Mala Agarwal. Tissue Culture of Momordica charantia L.: A Review. J. Plant Sci. 2014, 3(1-1), 24-32. doi: 10.11648/j.jps.s.2015030101.14
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Download@article{10.11648/j.jps.s.2015030101.14,
author = {Mala Agarwal},
title = {Tissue Culture of Momordica charantia L.: A Review},
journal = {Journal of Plant Sciences},
volume = {3},
number = {1-1},
pages = {24-32},
doi = {10.11648/j.jps.s.2015030101.14},
url = {https://doi.org/10.11648/j.jps.s.2015030101.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.s.2015030101.14},
abstract = {Plant tissue culture is the technique to culture plant cells or tissues under controlled aseptic conditions on a synthetic medium .It has value in basic research like cell biology, genetic transformation studies &biochemistry for the production of medicinally valuable secondary metabolites. Besides this also has commercial application. This review work outlines the work done on the tissue culture of Momordica charantia L. Momordica charantia L. commonly known as bitter melon/gourd, a member of Cucarbitaceae, is a slender, tendril climbing, annual vine. Bitter melon is a common food item of the tropics and is used for the treatment of cancer, diabetes, AIDS and many ailments. It is a potent hypoglycemic agent and its hypoglycemic actions for potential benefit in diabetes mellitus are possible due to at least three different groups of constituents in bitter melon. These include alkaloids, insulin like peptides, and a mixture of steroidal sapogenins known as charantin. Clinical studies with multiple controls have confirmed the benefit of bitter melon for diabetes. Alpha and beta momarcharin are two proteins found in bitter melon, which are known to inhibit the AIDS virus.},
year = {2014}
}
TY - JOUR T1 - Tissue Culture of Momordica charantia L.: A Review AU - Mala Agarwal Y1 - 2014/12/27 PY - 2014 N1 - https://doi.org/10.11648/j.jps.s.2015030101.14 DO - 10.11648/j.jps.s.2015030101.14 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 24 EP - 32 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.s.2015030101.14 AB - Plant tissue culture is the technique to culture plant cells or tissues under controlled aseptic conditions on a synthetic medium .It has value in basic research like cell biology, genetic transformation studies &biochemistry for the production of medicinally valuable secondary metabolites. Besides this also has commercial application. This review work outlines the work done on the tissue culture of Momordica charantia L. Momordica charantia L. commonly known as bitter melon/gourd, a member of Cucarbitaceae, is a slender, tendril climbing, annual vine. Bitter melon is a common food item of the tropics and is used for the treatment of cancer, diabetes, AIDS and many ailments. It is a potent hypoglycemic agent and its hypoglycemic actions for potential benefit in diabetes mellitus are possible due to at least three different groups of constituents in bitter melon. These include alkaloids, insulin like peptides, and a mixture of steroidal sapogenins known as charantin. Clinical studies with multiple controls have confirmed the benefit of bitter melon for diabetes. Alpha and beta momarcharin are two proteins found in bitter melon, which are known to inhibit the AIDS virus. VL - 3 IS - 1-1 ER -
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